Version 1
: Received: 15 November 2022 / Approved: 16 November 2022 / Online: 16 November 2022 (12:45:26 CET)
How to cite:
Perera, M.; Perera, I. Versertality of Bacteriome Dominated by Rothia mucilaginosa in a Group of Sri Lankan Oral Fibro Epithelial Polyp Male Patient: A Pilot Study. Preprints2022, 2022110308. https://doi.org/10.20944/preprints202211.0308.v1
Perera, M.; Perera, I. Versertality of Bacteriome Dominated by Rothia mucilaginosa in a Group of Sri Lankan Oral Fibro Epithelial Polyp Male Patient: A Pilot Study. Preprints 2022, 2022110308. https://doi.org/10.20944/preprints202211.0308.v1
Perera, M.; Perera, I. Versertality of Bacteriome Dominated by Rothia mucilaginosa in a Group of Sri Lankan Oral Fibro Epithelial Polyp Male Patient: A Pilot Study. Preprints2022, 2022110308. https://doi.org/10.20944/preprints202211.0308.v1
APA Style
Perera, M., & Perera, I. (2022). Versertality of Bacteriome Dominated by <em>Rothia mucilaginosa</em> in a Group of Sri Lankan Oral Fibro Epithelial Polyp Male Patient: A Pilot Study. Preprints. https://doi.org/10.20944/preprints202211.0308.v1
Chicago/Turabian Style
Perera, M. and Irosha Perera. 2022 "Versertality of Bacteriome Dominated by <em>Rothia mucilaginosa</em> in a Group of Sri Lankan Oral Fibro Epithelial Polyp Male Patient: A Pilot Study" Preprints. https://doi.org/10.20944/preprints202211.0308.v1
Abstract
The proportion of human cells to the microbial cell is 1:1. These procaryotes use efflux pumps and enzymes to prevent cellular intoxication of ions and compounds respectively. There is promising evidence on the role of the gut microbiome and its enzymes in metabolizing xenobiotics. The genetic potential of oral bacteria in drug and xenobiotic metabolism is yet to be unveiled. This study aimed to characterize the bacteriome associated with oral fibroepithelial polyps (FEP) and to predict the genetic potential. A representative sub-sample of 22 clinically diagnosed oral FEP (the control group) was selected from a main case-control study. Amplification of nucleotides of extracted DNA from frozen tissues was performed for the V1 to V3 region and sequencing of the amplicon with Illumina’s 2 X 300–bp chemistry. Classification of high-quality nonchimeric merged reads was done to the species level with a prioritized BLASTN-based algorithm. Downstream compositional analysis was performed with QIIME (Quantitative Insights into Microbial Ecology). Functional prediction of bacteriome was obtained by PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States). Rothia mucilaginosa, Streptococcus mitis, Gamella haemolysans, Streptococcus sp. oral taxon 431, and Rothia dentocariosa accounted for the top five taxa among 810 bacterial species according to the percentage of average relative abundance. Rothia mucilaginosa was elevated statistically significantly (p< 0.05). The genetic potential of xenobiotics and drug metabolism catalyzed by the P450 enzymes was observed for the first time as an attribute of bacteriome associated with oral FEP tissues dominated by R. mucilaginosa. This finding needs further investigation.
Medicine and Pharmacology, Dentistry and Oral Surgery
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The commenter has declared there is no conflict of interests.
Comment:
This pilot study provides an accidental finding, for the 1st time the genetic potential of xenobiotic metabolism was displayed by oral bacteriome consisting of a total of 810 bacterial species identified in FEP subjects, with inter-individual variations ranging from 23 to 229. Of them, Rothia mucilaginosa dominated, followed by Streptococcus mitis, Gamella haemolysans, Streptococcus sp. oral taxon 431, and Rothia dentocariosa present in more than 50% of individuals depending on their relative abundances. This finding needs confirm by a descriptive cross-sectional study with a large sample size of healthy individuals in an area, where farmers use agrochemicals. Isolation and identification of xenobiotic metabolizing bacteria from the oral cavity and identifying their specific enzymes using indicator media or chromogenic media would open up a fortune of industrial application of commensal oral bacteria for a particular purpose!
Commenter:
The commenter has declared there is no conflict of interests.