Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron Near Infrared Beam

Molong Han
,
Daniel Smith
,
Soon Hock Ng
ORCID logo ,
Tomas Katkus
,
Aravind Simon John Francis Rajeswary
,
Praveen P A
,
Keith R Bambery
ORCID logo ,
Mark J Tobin
,
Jitraporn Vongsvivut
ORCID logo ,
Saulius Juodkazis
,
Vijayakumar Anand
*
Version 1 : Received: 17 October 2022 / Approved: 18 October 2022 / Online: 18 October 2022 (08:28:25 CEST)

A peer-reviewed article of this Preprint also exists.

Han, M.; Smith, D.; Ng, S.H.; Katkus, T.; John Francis Rajeswary, A.S.; Praveen, P.A.; Bambery, K.R.; Tobin, M.J.; Vongsvivut, J.; Juodkazis, S.; Anand, V. Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam. Biosensors 2022, 12, 1073. Han, M.; Smith, D.; Ng, S.H.; Katkus, T.; John Francis Rajeswary, A.S.; Praveen, P.A.; Bambery, K.R.; Tobin, M.J.; Vongsvivut, J.; Juodkazis, S.; Anand, V. Single Shot Lensless Interferenceless Phase Imaging of Biochemical Samples Using Synchrotron near Infrared Beam. Biosensors 2022, 12, 1073.

Abstract

Phase imaging of biochemical samples has been demonstrated for the first time at the Infrared Microspectroscopy (IRM) beamline of the Australian Synchrotron using the usually discarded Near-IR (NIR) region of the synchrotron-IR beam. The synchrotron-IR beam at the Australian Synchrotron IRM beamline has a unique fork shaped intensity distribution as a result of the gold coated extraction mirror shape, which includes a central slit for rejection of the intense X-ray beam. The resulting beam configuration makes any imaging task challenging. For intensity imaging, the fork shaped beam is usually tightly focused to a point on the sample plane followed by a pixel-by-pixel scanning approach to record the image. In this study, a pinhole was aligned with one of the lobes of the fork shaped beam and the Airy diffraction pattern was used to illuminate biochemical samples. The diffracted light from the samples was captured using a NIR sensitive lensless camera. A rapid phase-retrieval algorithm was applied to the recorded intensity distributions to reconstruct the phase information corresponding to different planes. The preliminary results are promising to develop multimodal imaging capabilities at the IRM beamline of the Australian Synchrotron.

Keywords

Phase imaging, bioimaging; synchrotron; near infrared beam; holography; incoherent optics; chemical imaging; phase retrieval; 3D imaging.

Subject

Physical Sciences, Optics and Photonics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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