Version 1
: Received: 25 August 2022 / Approved: 30 August 2022 / Online: 30 August 2022 (10:30:06 CEST)
How to cite:
Mohamed, W.S.E.; Aldabbagh, H.A.; Aldughmani, H.A.; Abdullah, Z.F.; Kassem, S.A.; Abdel-Almoniem, I.A.; Ibrahim, M.F. Prevalence of Human Herpesviruses (HHV1-7), and its Clinical Significance in Middle Eastern Pediatric Leukemia Patients Using 2 Independent PCR Assays. Preprints2022, 2022080520. https://doi.org/10.20944/preprints202208.0520.v1
Mohamed, W.S.E.; Aldabbagh, H.A.; Aldughmani, H.A.; Abdullah, Z.F.; Kassem, S.A.; Abdel-Almoniem, I.A.; Ibrahim, M.F. Prevalence of Human Herpesviruses (HHV1-7), and its Clinical Significance in Middle Eastern Pediatric Leukemia Patients Using 2 Independent PCR Assays. Preprints 2022, 2022080520. https://doi.org/10.20944/preprints202208.0520.v1
Mohamed, W.S.E.; Aldabbagh, H.A.; Aldughmani, H.A.; Abdullah, Z.F.; Kassem, S.A.; Abdel-Almoniem, I.A.; Ibrahim, M.F. Prevalence of Human Herpesviruses (HHV1-7), and its Clinical Significance in Middle Eastern Pediatric Leukemia Patients Using 2 Independent PCR Assays. Preprints2022, 2022080520. https://doi.org/10.20944/preprints202208.0520.v1
APA Style
Mohamed, W.S.E., Aldabbagh, H.A., Aldughmani, H.A., Abdullah, Z.F., Kassem, S.A., Abdel-Almoniem, I.A., & Ibrahim, M.F. (2022). Prevalence of Human Herpesviruses (HHV1-7), and its Clinical Significance in Middle Eastern Pediatric Leukemia Patients Using 2 Independent PCR Assays. Preprints. https://doi.org/10.20944/preprints202208.0520.v1
Chicago/Turabian Style
Mohamed, W.S.E., Iman Adel Abdel-Almoniem and Mohamed Fawzy Ibrahim. 2022 "Prevalence of Human Herpesviruses (HHV1-7), and its Clinical Significance in Middle Eastern Pediatric Leukemia Patients Using 2 Independent PCR Assays" Preprints. https://doi.org/10.20944/preprints202208.0520.v1
Abstract
Objectives: Human herpes viruses can cause life-threatening diseases in immunocompromised children, especially leukemic patients. Therefore, the aim of this study is to detect the human herpes viruses (HHV1-7) and to investigate its clinical significance in Middle Eastern Pediatric Leukemia Patients by using 2 Independent PCR assays. Methods: Detection of human herpes virus DNA has been done in blood samples of 200 pediatric leukemia patients in addition to 90 blood donors as a control group using multiplex PCR assays. When a ‘‘positive’’ result was observed, real-time PCR was performed to measure the viral load. Results: The most frequent herpes virus infection in Middle Eastern Pediatric Leukemia cases was CMV, followed by EBV, then HHV6, VZV, HHV7, HSV1, and HSV2, where they were 92/200 (46%), 76/200 (38%), 72/200 (36%), 48/200 (24%), 12/200 (6%), 8/200 (4%), and 2/200 (1%) respectively. Also, there was a statistically significance difference between leukemic patients and their controls regarding CMV, EBV, HHV6, and VZV (P <0.05). Correlation between percentage of co-infection, and clinical parameters for the 7 herpes viruses has been studied, and there is an increase in absolute neutrophilic count (ANC), total leukocyte count (TLC) and duration of fever and neutropenia in age group 6-11 years for HHV6/CMV, then in age group 12-18 years especially for EBV/CMV and CMV/HHV6. Also, our results show that multiplex PCR assay is close to single PCR assay in relation to specificity and sensitivity which in turn prove its validity for early diagnosis of herpes viral infection. Conclusions: Adopting multiplex PCR technique is helpful in screening of virus infections. It will save time, effort, cost effective and will assist in rapid diagnosis. However, the clinical relevance of the virus infection needs to be evaluated by quantitative real-time PCR which in turn will help patient's management by using appropriate antiviral treatment.
Keywords
Multiplex PCR; Real time PCR; Human herpes viruses; clinical significance; Pediatric Leukemia patients
Subject
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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