Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Novel Black Pepper Antinematode Compounds Produced by Bacillus velezensis RB.EK7 Conversion of Organic Wastes

Thi Huyen Trang Trinh
,
San-Lang Wang
* ORCID logo ,
Van Bon Nguyen
* ORCID logo ,
Tu Quy Phan
,
Manh Dung Doan
ORCID logo ,
Thi Phuong Hanh Tran
,
Thi Huyen Nguyen
,
Thi Anh Hong Le
,
That Quang Ton
,
Anh Dzung Nguyen
*
Version 1 : Received: 6 August 2022 / Approved: 8 August 2022 / Online: 8 August 2022 (10:30:28 CEST)

A peer-reviewed article of this Preprint also exists.

Trinh, T.H.T.; Wang, S.-L.; Nguyen, V.B.; Phan, T.Q.; Doan, M.D.; Tran, T.P.H.; Nguyen, T.H.; Le, T.A.H.; Ton, T.Q.; Nguyen, A.D. Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by Bacillus velezensis RB.EK7 against Black Pepper Nematodes. Agronomy 2022, 12, 2300. Trinh, T.H.T.; Wang, S.-L.; Nguyen, V.B.; Phan, T.Q.; Doan, M.D.; Tran, T.P.H.; Nguyen, T.H.; Le, T.A.H.; Ton, T.Q.; Nguyen, A.D. Novel Nematocidal Compounds from Shrimp Shell Wastes Valorized by Bacillus velezensis RB.EK7 against Black Pepper Nematodes. Agronomy 2022, 12, 2300.

Abstract

Bacillus veleznesis RB.EK7 was recently found as a potent rhizobacterial strain for effective management of black pepper root-knot nematodes. This work aimed to produce, purify, and elucidate the chemical structures of antinematode compounds (ANCs). Concerning cost-effectiveness and environmental issues, this study used organic wastes for the bioproduction of ANCs. Among various substrates, shrimp shells powder was the most suitable carbon/nitrogen source to produce ANCs. The fermentation process for enhancement of antinematode activity was investigated. The targeting ANCs were purified from the fermented culture broth, and their structures were elucidated. Two active compounds were thymine (1) and hexahydropyrrolo [1,2-a]pyrazine-1,4-dione (2). Notably, for the first time, these purified compounds showed potential and moderate anti- J2 nematodes and anti-eggs hatching, respectively. The docking study results indicated that the potent antinematode effect of these compounds may be possibly due to the inhibition of the targeting enzyme acetylcholinesterase. The data of this work suggest that organic waste SSP can be potentially reused for the production of thymine and hexahydropyrrolo [1,2-a] pyrazine-1,4-dione with promising use for the management of black pepper nematodes.

Keywords

black pepper; Bacillus veleznesis; root-knot nematodes; antinematodes compounds; organic wastes; microbial fermentation; thymine; hexahydropyrrolo [1,2-a]pyrazine-1,4-dione

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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