Calderón-Montaño, J.M.; Guillén-Mancina, E.; Jiménez-Alonso, J.J.; Jiménez-González, V.; Burgos-Morón, E.; Mate, A.; Pérez-Guerrero, M.C.; López-Lázaro, M. Manipulation of Amino Acid Levels With Artificial Diets Induces Anticancer Activity in Mice With Renal Cell Carcinoma. Preprints2022, 2022080088. https://doi.org/10.20944/preprints202208.0088.v1
APA Style
Calderón-Montaño, J.M., Guillén-Mancina, E., Jiménez-Alonso, J.J., Jiménez-González, V., Burgos-Morón, E., Mate, A., Pérez-Guerrero, M.C., & López-Lázaro, M. (2022). Manipulation of Amino Acid Levels With Artificial Diets Induces Anticancer Activity in Mice With Renal Cell Carcinoma. Preprints. https://doi.org/10.20944/preprints202208.0088.v1
Chicago/Turabian Style
Calderón-Montaño, J.M., María Concepción Pérez-Guerrero and Miguel López-Lázaro. 2022 "Manipulation of Amino Acid Levels With Artificial Diets Induces Anticancer Activity in Mice With Renal Cell Carcinoma" Preprints. https://doi.org/10.20944/preprints202208.0088.v1
Abstract
Targeted therapies with antiangiogenic drugs (e.g., sunitinib) and immune checkpoint inhibitors (e.g., anti-PD-1 antibodies) are the standard of care for patients with metastatic renal cell carcinoma. Although these treatments improve patient survival, they are rarely curative. We previously hypothesized that advanced cancers might be treated without drugs by using artificial diets in which the levels of specific amino acids (AAs) are manipulated. In this work, after showing that AA manipulation induces selective anticancer activity in renal cell carcinoma cells in vitro, we evaluated the anticancer activity of 17 artificial diets in a challenging animal model of renal cell carcinoma. The model was stablished by injecting murine renal cell carcinoma (Renca) cells into the peritoneum of immunocompetent BALB/cAnNRj mice. Mice survival was markedly improved when their normal diet was replaced with our artificial diets. Mice fed a diet lacking six AAs (diet T2) lived longer than mice treated with sunitinib or anti-PD-1 immunotherapy; several animals lived very long or were cured. Controlling the levels of several AAs (e.g., cysteine, methionine and leucine) and lipids was important for the anticancer activity of the diets. Additional studies are needed to further evaluate the therapeutic potential of this simple and inexpensive anticancer strategy.
Keywords
Amino acids; cancer; cancer metabolism; cancer therapy; kidney cancer; renal adenocarcinoma; renal cancer; metastasis; selective amino acid restriction therapy; restriction
Subject
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Commenter:
The commenter has declared there is no conflict of interests.
Journal reference: International Journal of Molecular Sciences 2022
DOI: 10.3390/ijms232416132