PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Dysregulated Expression of Ion Channels in Sensory Neurons Enhances Action Potential Afterhyperpolarization in Patients with Chronic Kidney Disease–Associated Pruritus
Version 1
: Received: 29 July 2022 / Approved: 29 July 2022 / Online: 29 July 2022 (04:50:39 CEST)
How to cite:
Momose, A.; Yabe, M.; Chiba, S.; Kumakawa, K.; Shiraiwa, Y.; Mizukami, H. Dysregulated Expression of Ion Channels in Sensory Neurons Enhances Action Potential Afterhyperpolarization in Patients with Chronic Kidney Disease–Associated Pruritus. Preprints2022, 2022070449. https://doi.org/10.20944/preprints202207.0449.v1
Momose, A.; Yabe, M.; Chiba, S.; Kumakawa, K.; Shiraiwa, Y.; Mizukami, H. Dysregulated Expression of Ion Channels in Sensory Neurons Enhances Action Potential Afterhyperpolarization in Patients with Chronic Kidney Disease–Associated Pruritus. Preprints 2022, 2022070449. https://doi.org/10.20944/preprints202207.0449.v1
Momose, A.; Yabe, M.; Chiba, S.; Kumakawa, K.; Shiraiwa, Y.; Mizukami, H. Dysregulated Expression of Ion Channels in Sensory Neurons Enhances Action Potential Afterhyperpolarization in Patients with Chronic Kidney Disease–Associated Pruritus. Preprints2022, 2022070449. https://doi.org/10.20944/preprints202207.0449.v1
APA Style
Momose, A., Yabe, M., Chiba, S., Kumakawa, K., Shiraiwa, Y., & Mizukami, H. (2022). Dysregulated Expression of Ion Channels in Sensory Neurons Enhances Action Potential Afterhyperpolarization in Patients with Chronic Kidney Disease–Associated Pruritus. Preprints. https://doi.org/10.20944/preprints202207.0449.v1
Chicago/Turabian Style
Momose, A., Yasuo Shiraiwa and Hiroki Mizukami. 2022 "Dysregulated Expression of Ion Channels in Sensory Neurons Enhances Action Potential Afterhyperpolarization in Patients with Chronic Kidney Disease–Associated Pruritus" Preprints. https://doi.org/10.20944/preprints202207.0449.v1
Abstract
Expression levels of Cav3.2, BKCa, and anoctamin 1 were previously found to be significantly elevated in patients with chronic kidney disease–associated pruritus (CKD-aP). On the other hand, the expression of TRPV1 was significantly reduced. We further compared CKD patients with and without CKD-aP in terms of the expression levels of several ion channels in the skin, including in peripheral nerve endings。Based on CKD-aP severity, subjects were divided into two groups: non-CKD-aP (no or slight pruritus; n = 16) and CKD-aP (mild, moderate, or severe pruritus; n = 16). Skin samples were obtained from the forearm or elbow during arteriovenous fistula surgery. We used quantitative real-time polymerase chain reaction to measure the skin expression levels of the following ion channels in the skin: Nav1.7, Kv7.2, TREK1, HCN2, TrkA, and Piezo2. RT-PCR analyses showed that CKD-aP patients had significantly higher levels of TREK1 and Piezo2 transcripts and significantly lower levels of HCN2 transcripts than non–CKD-aP patients. No significant differences were noted between groups in the expression of Nav1.7 or TrkA. Moreover, Kv7.2 transcripts were not detected in either group. In skin samples collected from CKD-aP patients, ion channel expression patterns were altered to enhance hyperpolarization of pruriceptive neurons.
Keywords
CKD-aP; ion channels; action potential; hyperpolarization; RT-PCR
Subject
Medicine and Pharmacology, Dermatology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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