Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Prevalence of Hepatitis B Virus Mutations Associated with Hepatocellular Carcinoma in HBV and HIV Co-infected Adults in Botswana

Version 1 : Received: 30 June 2022 / Approved: 1 July 2022 / Online: 1 July 2022 (16:31:00 CEST)

How to cite: Anderson, M.; Choga, W.T.; Phinius, B.B.; Bhebhe, L.N.; Gotulweng, S.; Baruti, K.; Moyo, S.; Sebunya, T.K.; Musonda, R.M.; Blackard, J.T.; Gaseitsiwe, S. Prevalence of Hepatitis B Virus Mutations Associated with Hepatocellular Carcinoma in HBV and HIV Co-infected Adults in Botswana. Preprints 2022, 2022070018 (doi: 10.20944/preprints202207.0018.v1). Anderson, M.; Choga, W.T.; Phinius, B.B.; Bhebhe, L.N.; Gotulweng, S.; Baruti, K.; Moyo, S.; Sebunya, T.K.; Musonda, R.M.; Blackard, J.T.; Gaseitsiwe, S. Prevalence of Hepatitis B Virus Mutations Associated with Hepatocellular Carcinoma in HBV and HIV Co-infected Adults in Botswana. Preprints 2022, 2022070018 (doi: 10.20944/preprints202207.0018.v1).

Abstract

Mutations within the hepatitis B virus (HBV) genome have been associated with rapid progres-sion to hepatocellular carcinoma (HCC); however, there is limited information regarding the prevalence and impact of these mutations in most of sub-Saharan Africa, including Botswana. We aimed to determine the prevalence of HBV mutations known to be associated with progression to HCC using a retrospective, cross-sectional analysis of 48 previously generated HBV sequences from adults with concomitant HBV/HIV initiating HIV antiretroviral therapy in Botswana. The sequences were aligned with reference sequences, and HCC-associated mutations were manually identified using BioEdit. Sixteen (33.3 %) of 48 participant samples had 20 HCC-associated mu-tations. Seven HCC mutations were present in the core region, 4 in the preCore region, 7 in the X region, and one mutation in the surface region, as well as deletions within the preSurface 1 region. Seven of the 16 participants (43.8%) had multiple HCC-associated mutations. There were also previously uncharacterized mutations at positions with known HCC-associated mutations. HCC-associated mutations were common in this cohort; hence, some participants may require close clinical monitoring as they might be more prone to rapid disease progression. Other functionally uncharacterized polymorphisms were also detected and require characterization in future studies.

Keywords

hepatocellular carcinoma (HCC); hepatitis B virus (HBV); mutations; HBV/HIV co-infection; Botswana; Africa

Subject

LIFE SCIENCES, Virology

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