Version 1
: Received: 21 June 2022 / Approved: 22 June 2022 / Online: 22 June 2022 (11:50:20 CEST)
How to cite:
Bochenek, H.; Francis, N.; Santhakumar, A.B.; Blanchard, C.L.; Chinkwo, K.A. Chickpea Water and Chickpea Polyphenols Induce Apoptosis and Alleviate Cell Migration In Vitro in Human Colon Adenocarcinoma Cells. Preprints2022, 2022060317. https://doi.org/10.20944/preprints202206.0317.v1
Bochenek, H.; Francis, N.; Santhakumar, A.B.; Blanchard, C.L.; Chinkwo, K.A. Chickpea Water and Chickpea Polyphenols Induce Apoptosis and Alleviate Cell Migration In Vitro in Human Colon Adenocarcinoma Cells. Preprints 2022, 2022060317. https://doi.org/10.20944/preprints202206.0317.v1
Bochenek, H.; Francis, N.; Santhakumar, A.B.; Blanchard, C.L.; Chinkwo, K.A. Chickpea Water and Chickpea Polyphenols Induce Apoptosis and Alleviate Cell Migration In Vitro in Human Colon Adenocarcinoma Cells. Preprints2022, 2022060317. https://doi.org/10.20944/preprints202206.0317.v1
APA Style
Bochenek, H., Francis, N., Santhakumar, A.B., Blanchard, C.L., & Chinkwo, K.A. (2022). Chickpea Water and Chickpea Polyphenols Induce Apoptosis and Alleviate Cell Migration In Vitro in Human Colon Adenocarcinoma Cells. Preprints. https://doi.org/10.20944/preprints202206.0317.v1
Chicago/Turabian Style
Bochenek, H., Christopher L. Blanchard and Kenneth A. Chinkwo. 2022 "Chickpea Water and Chickpea Polyphenols Induce Apoptosis and Alleviate Cell Migration In Vitro in Human Colon Adenocarcinoma Cells" Preprints. https://doi.org/10.20944/preprints202206.0317.v1
Abstract
Chickpea is an essential legume, a staple food in many cultures and contains nutrients with potential health benefits. The chickpea water (CPW) leached out after cooking is usually discarded, which may potentially have significant anti-cancer and other health beneficial properties. This study compared the in-vitro bioactivity of CPW with chickpea polyphenol extract (CPPE) to evaluate its impact on pathways of colorectal cancer progression and development. Morphological observation by APOPercentage, cell viability detection using a cytotoxic assay and cell migration-scratch assay points to measure rate of metastasis were employed. Overall antioxidant activity of CPW and CPPE were measured using ABTS and DPPH free-radical assays. At 50 µg/mL concentration and above, both CPW and CPPE extracts significantly reduce cell viability in HT-29 colon cancer cell lines (p < 0.05). Moreover, a quantitative analysis of the extent of apoptosis demonstrated that at 250 and 500 μg/mL concentrations, both extracts induced significant apoptosis compared to the untreated control. Meanwhile, the cell migration scratch area decreases by 34.42% and 15.27% when treated with CPW and CPPE, respectively. In summary, CPW demonstrated comparable in vitro anti-cancer properties and antioxidant activity in colorectal cancer cells to CPPE. Further, in vivo studies are warranted to evaluate the physiological bioactivity of CPW and CPPE in targeting pathways of cancer development and progression
Keywords
chick pea; chick pea water; apoptosis; cytotoxicity; colorectal cancer; polyphenols
Subject
Biology and Life Sciences, Aging
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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