Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Indicated Role of Small Cellular Particles in Mediating Inter-Species Interaction for Removal of Bisphenols from Conditioned Media

David Skufca
,
Darja Bozic
,
Matej Hocevar
,
Marko Jeran
ORCID logo ,
Apolonija Bedina Zavec
,
Matic Kisovec
ORCID logo ,
Marjetka Podobnik
ORCID logo ,
Rok Tomazin
,
Tadeja Matos
,
Ales Iglic
ORCID logo ,
Tjasa Griessler Bulc
,
Ester Heath
,
Veronika Kralj-Iglic
*
Version 1 : Received: 16 June 2022 / Approved: 17 June 2022 / Online: 17 June 2022 (03:57:22 CEST)

A peer-reviewed article of this Preprint also exists.

Škufca, D.; Božič, D.; Hočevar, M.; Jeran, M.; Bedina Zavec, A.; Kisovec, M.; Podobnik, M.; Matos, T.; Tomazin, R.; Iglič, A.; Griessler Bulc, T.; Heath, E.; Kralj-Iglič, V. Interaction between Microalgae P. tricornutum and Bacteria Thalassospira sp. for Removal of Bisphenols from Conditioned Media. Int. J. Mol. Sci. 2022, 23, 8447. Škufca, D.; Božič, D.; Hočevar, M.; Jeran, M.; Bedina Zavec, A.; Kisovec, M.; Podobnik, M.; Matos, T.; Tomazin, R.; Iglič, A.; Griessler Bulc, T.; Heath, E.; Kralj-Iglič, V. Interaction between Microalgae P. tricornutum and Bacteria Thalassospira sp. for Removal of Bisphenols from Conditioned Media. Int. J. Mol. Sci. 2022, 23, 8447.

Abstract

Small cellular particles are released into the surroundings of cells and are proposed to play an important role in intercellular communication and consequently the responses of microbial com-munities to environmental stressors. We studied the connection between the small cellular parti-cles and the efficiency of three culture series of the microalge Phaeodactylum tricornutum and bac-teria (axenic microalgae, bacterial culture and co-culture of the two) in removing bisphenols from their growth medium. The microorganism growth rate was determined by flow cytometry, protein profiles were examined by protein gel electrophoresis, cultures and small cellular particle isolates were imaged by scanning electron microscopy, and bisphenols were analyzed using gas chroma-tography coupled with tandem mass spectrometry (GC-MS/MS). Higher growth rates of microal-gae were observed in the co-culture than in the axenic microalgal culture, while the presence of bisphenols neither influenced the morphology of the microalgal cells, protein profiles, nor the small cellular particle isolates. Biotic removal of bisphenols ranged from 0% to 71% and differed among the culture series in a compound-specific manner. However, it remains unclear which mechanisms influenced algal growth and bisphenol removal. Further research on the mechanisms of interspecies communication is needed to advance our understanding of microbial communities at the nano-level.

Keywords

contaminants of emerging concern; bisphenol; microalgae; Phaeodactylum tricornutum; bacteria, extracellular vesicles; electron microscopy; flow cytometry; mass spectrometry

Subject

Environmental and Earth Sciences, Environmental Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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