Komine, R.; Sugasawa, T.; Manevich, L.; Tamai, S.; Takekoshi, K.; Kanki, Y. Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle. Preprints2022, 2022050083. https://doi.org/10.20944/preprints202205.0083.v1
Komine, R., Sugasawa, T., Manevich, L., Tamai, S., Takekoshi, K., & Kanki, Y. (2022). Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle. Preprints. https://doi.org/10.20944/preprints202205.0083.v1
Komine, R., Kazuhiro Takekoshi and Yasuharu Kanki. 2022 "Gene Expression Profile Provides Novel Insights of Fasting-Refeeding Response in Zebrafish Skeletal Muscle" Preprints. https://doi.org/10.20944/preprints202205.0083.v1
Recently, fasting has been spotlighted from a healthcare perspective. However, the de-tailed biological mechanisms and significance by which the effects of fasting confer health benefits are not yet clear. Due to certain advantages of zebrafish, as a vertebrate model widely utilized in biological studies, we used mRNA-sequencing and bioinformatics analysis to examine comprehensive gene expression changes in skeletal muscle tissues during fasting-refeeding. Our results produced a novel set of nutrition-related genes under a fasting-refeeding protocol. We found five dramatically upregulated genes in each fasting (for 24 hours) and refeeding (after 3 hours), exhibiting a rapid response to the provided conditional changes. The assessment of the gene length revealed, the gene set whose expression was elevated only after 3 hours of refeeding had a shorter length, suggesting that nutrition-related gene function is associated with gene length. Taken together, our results from bioinformatics analyses provide new insights into biological mechanisms induced by fasting-refeeding conditions within zebrafish skeletal muscle.
Biology and Life Sciences, Biochemistry and Molecular Biology
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