Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Flexible Multiplane Structured Illumination Microscope With a Four-Camera Detector

Version 1 : Received: 29 April 2022 / Approved: 4 May 2022 / Online: 4 May 2022 (12:24:22 CEST)

A peer-reviewed article of this Preprint also exists.

Johnson, K.A.; Noble, D.; Machado, R.; Paul, T.C.; Hagen, G.M. Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector. Photonics 2022, 9, 501. Johnson, K.A.; Noble, D.; Machado, R.; Paul, T.C.; Hagen, G.M. Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector. Photonics 2022, 9, 501.

Journal reference: Photonics 2022, 9, 501
DOI: 10.3390/photonics9070501

Abstract

Fluorescence microscopy provides an unparalleled tool for imaging biological samples. However, producing high-quality volumetric images quickly and without excessive complexity remains a challenge. Here, we demonstrate a simple multi-camera structured illumination microscope (SIM) capable of simultaneously imaging multiple focal planes, allowing for the capture of 3D fluorescent images without any axial movement of the sample. This simple setup allows for the acquisition of many different 3D imaging modes, including 3D time lapses, high-axial-resolution 3D images, and large 3D mosaics.

Keywords

structured illumination; fluorescence; brain; multi-camera

Subject

LIFE SCIENCES, Biophysics

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