Halfon, P.; Jordana, S.; Blachier, S.; Cartlamy, P.; Kbaier, L.; Psomas, C.K.; Philibert, P.; Antoniotti, G.; Allemand-Sourrieu, J.; Rebaudet, S.; et al. Anti-Spike Protein to Determine SARS-CoV-2 Antibody Levels: Is There a Specific Threshold Conferring Protection in Immunocompromised Patients? PLOS ONE 2023, 18, e0281257, doi:10.1371/journal.pone.0281257.
Halfon, P.; Jordana, S.; Blachier, S.; Cartlamy, P.; Kbaier, L.; Psomas, C.K.; Philibert, P.; Antoniotti, G.; Allemand-Sourrieu, J.; Rebaudet, S.; et al. Anti-Spike Protein to Determine SARS-CoV-2 Antibody Levels: Is There a Specific Threshold Conferring Protection in Immunocompromised Patients? PLOS ONE 2023, 18, e0281257, doi:10.1371/journal.pone.0281257.
Halfon, P.; Jordana, S.; Blachier, S.; Cartlamy, P.; Kbaier, L.; Psomas, C.K.; Philibert, P.; Antoniotti, G.; Allemand-Sourrieu, J.; Rebaudet, S.; et al. Anti-Spike Protein to Determine SARS-CoV-2 Antibody Levels: Is There a Specific Threshold Conferring Protection in Immunocompromised Patients? PLOS ONE 2023, 18, e0281257, doi:10.1371/journal.pone.0281257.
Halfon, P.; Jordana, S.; Blachier, S.; Cartlamy, P.; Kbaier, L.; Psomas, C.K.; Philibert, P.; Antoniotti, G.; Allemand-Sourrieu, J.; Rebaudet, S.; et al. Anti-Spike Protein to Determine SARS-CoV-2 Antibody Levels: Is There a Specific Threshold Conferring Protection in Immunocompromised Patients? PLOS ONE 2023, 18, e0281257, doi:10.1371/journal.pone.0281257.
Abstract
Background: Identifying a specific threshold level of SARS-CoV-2 antibodies that confers protection in immunocompromised patients has been very challenging. The aim was to assess the threshold of 264 binding antibody units (BAU)/ml using four different SARS-CoV-2 antibody assays (Abbott, Beckman, Roche, and Siemens) and to establish a new optimal threshold of protection for each of the four antibody assays. Methods: This study was performed on data retrieved from 69 individuals, who received at least one dose of the Pfizer/BioNTech BNT162b2 or Moderna COVID-19 vaccine (Spikevax) at the Alphabio Laboratory in Marseille, France (European Hospital, Alphabio – Biogroup). The results were compared to the percent inhibition calculated using a functional surrogate of a standardized virus neutralization test (Genscript). Results: Samples from 69 patients were analyzed. For a reference cutoff of 264 BAU/ml, assays showed moderate to good overall concordance with Genscript: 87% concordance for Abbott, 78% for Beckman, 75% for Roche, and 88% for Siemens. Overall concordance increased consistently after applying new thresholds, i.e., 148 BAU/ml (Abbott), 48 (Beckman), 559 (Roche), and 270 (Siemens). Conclusion: We suggest specific adjusted thresholds (BAU/ml) for the four commercial antibody assays that are used to assess pre-exposure prophylaxis in immunocompromised patients.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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