Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Characterization of Hantavirus N Protein Intracellular Dynamics and Localization

Robert-William Welke
,
Hannah Sabeth Sperber
,
Amit Koikkarah
,
Laura Menke
,
Christian Sieben
ORCID logo ,
Detlev Herbert Krüger
,
Salvatore Chiantia
ORCID logo ,
Andreas Herrmann
ORCID logo ,
Roland Schwarzer
*
Version 1 : Received: 2 January 2022 / Approved: 6 January 2022 / Online: 6 January 2022 (11:45:52 CET)

A peer-reviewed article of this Preprint also exists.

Welke, R.-W.; Sperber, H.S.; Bergmann, R.; Koikkarah, A.; Menke, L.; Sieben, C.; Krüger, D.H.; Chiantia, S.; Herrmann, A.; Schwarzer, R. Characterization of Hantavirus N Protein Intracellular Dynamics and Localization. Viruses 2022, 14, 457. Welke, R.-W.; Sperber, H.S.; Bergmann, R.; Koikkarah, A.; Menke, L.; Sieben, C.; Krüger, D.H.; Chiantia, S.; Herrmann, A.; Schwarzer, R. Characterization of Hantavirus N Protein Intracellular Dynamics and Localization. Viruses 2022, 14, 457.

Abstract

Hantaviruses are enveloped viruses that possess a tri-segmented, negative-sense RNA genome. The viral S-segment encodes the multifunctional nucleocapsid protein (N), which is involved in genome packaging, intracellular protein transport, immunoregulation and several other crucial processes during hantavirus infection. In this study we have generated fluorescently tagged N protein constructs derived from Puumalavirus, the dominant hantavirus species in Central, Northern and Eastern Europe. We have comprehensively characterized this protein in the rodent cell line CHO-K1, monitoring the dynamics of N protein complex formation and investigating co-localization with host proteins as well as the viral glycoproteins Gc and Gn. We found a significant spatial correlation of N with vimentin, actin and P-bodies, but not with microtubules. N constructs also co-localized with Gn and Gc, albeit not as strong as the glycoproteins associated with each other. Moreover, we as-sessed oligomerization of N constructs, observing efficient and concentration-dependent multi-merization, with complexes comprising more than 10 individual proteins.

Keywords

Hantavirus; N protein; oligomerization; actin; P-Bodies; vimentin; Number&Brightness; Puumalavirus; macromolecular assemblies

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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