Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Organization of the Pig T-cell Receptor Gamma (TRG) Locus Provides Insights Into the Evolutionary Patterns of the TRG Genes Across Cetartiodactyla

Version 1 : Received: 21 December 2021 / Approved: 23 December 2021 / Online: 23 December 2021 (10:02:37 CET)

A peer-reviewed article of this Preprint also exists.

Linguiti, G.; Giannico, F.; D’Addabbo, P.; Pala, A.; Caputi Jambrenghi, A.; Ciccarese, S.; Massari, S.; Antonacci, R. The Organization of the Pig T-Cell Receptor γ (TRG) Locus Provides Insights into the Evolutionary Patterns of the TRG Genes across Cetartiodactyla. Genes 2022, 13, 177. Linguiti, G.; Giannico, F.; D’Addabbo, P.; Pala, A.; Caputi Jambrenghi, A.; Ciccarese, S.; Massari, S.; Antonacci, R. The Organization of the Pig T-Cell Receptor γ (TRG) Locus Provides Insights into the Evolutionary Patterns of the TRG Genes across Cetartiodactyla. Genes 2022, 13, 177.

Journal reference: Genes 2022, 13, 177
DOI: 10.3390/genes13020177

Abstract

The domestic pig (Sus scrofa) is a species representative of the Suina, one of the four suborders within Cetartiodactyla. In this paper, we reported our analysis of the pig TRG locus in comparison with the loci of species representative of the Ruminantia, Tylopoda and Cetacea suborders. The pig TRG genomic structure reiterates the peculiarity of the organization of Cetartiodactyla loci in TRGC “cassettes”, each containing the basic V-J-J-C unit. Eighteen genes arranged in four TRGC cassettes, form the pig TRG locus. All the functional TRG genes were expressed, and the TRGV genes preferentially rearrange with the TRGJ genes within their own cassette, which correlates the diversity of the gamma-chain repertoire with the number of cassettes. Among them, the TRGC5, located at the 5’ end of the locus, is the only cassette that retains a marked homology with the corresponding TRGC cassettes of all the analyzed species. The preservation of the TRGC5 cassette for such a long evolutionary time presumes a highly specialized function of its genes, which could be essential for the survival of species. Therefore, the maintenance of this cassette in pigs confirms that it is the most evolutionarily ancient within Cetartiodactyla, and it has undergone a process of duplication to give rise to the other TRGC cassettes in the different artiodactyl species in a lineage-specific manner.

Keywords

pig genome; gamma/delta T-cell; TRG locus; TRG genes; gamma/delta high species; Cetartiodac-tyla; Immunogenomics; evolution

Subject

LIFE SCIENCES, Genetics

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.

We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.