Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genomic Regions Influencing Preharvest Sprouting Tolerance in Two Doubled-Haploid Wheat Populations (Triticum aestivum L.)

Version 1 : Received: 18 December 2021 / Approved: 21 December 2021 / Online: 21 December 2021 (14:13:40 CET)

How to cite: Khumalo, T.P.; Hlongoane, T.; Barnard, A.; Tsilo, T.J. Genomic Regions Influencing Preharvest Sprouting Tolerance in Two Doubled-Haploid Wheat Populations (Triticum aestivum L.). Preprints 2021, 2021120348 (doi: 10.20944/preprints202112.0348.v1). Khumalo, T.P.; Hlongoane, T.; Barnard, A.; Tsilo, T.J. Genomic Regions Influencing Preharvest Sprouting Tolerance in Two Doubled-Haploid Wheat Populations (Triticum aestivum L.). Preprints 2021, 2021120348 (doi: 10.20944/preprints202112.0348.v1).

Abstract

The current and projected climate change that is represented by increasing temperatures, humidity levels and irregular rainfall patterns, promotes the occurrence of preharvest sprouting (PHS) in wheat. PHS results in significant economic losses, globally, which necessitates the need for high-yielding cultivars with increased PHS tolerance, hence this study was conducted. The current study evaluated two doubled-haploid (DH) wheat populations of Tugela-Dn × Elands and Elands × Flamink across six environments in the Free State Province of South Africa to select genotypes with increased PHS tolerance and further map the underlying loci. Significant effects of DH lines (194) and environments (6) were observed for PHS tolerance. The results of this study validate previous findings that PHS is only expressed when environmental conditions are conducive. Quantitative trait loci (QTL) mapping using single nucleotide polymorphism (SNP) and silicoDArT markers revealed three additive QTL with major effects on chromosomes 5B and 7B, and these QTL were detected more than once, when conditions were favourable. These QTL explained a phenotypic variation (PVE) varying between 10.08% and 20.30% (LOD = 2.73 – 3.11). About 16.50% of DH lines performed to the level of Elands (the PHS-tolerant parent) and are recommended for further selection in a pre-breeding or breeding programme. The findings of the study are expected to facilitate the on-going breeding efforts for PHS tolerance in winter wheat.

Keywords

phenotypic selection; preharvest sprouting tolerance; QTL mapping analysis; silicoDArT; SNP; wheat

Subject

LIFE SCIENCES, Genetics

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