Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Microfluidic Aptamer-Based Sensor for Detection of Mercury (II) and Lead (II) Ions in Water

Version 1 : Received: 2 September 2021 / Approved: 3 September 2021 / Online: 3 September 2021 (10:29:50 CEST)

How to cite: Huang, W.; Wu, R.; Yeh, K.; Mai, V.; Yang, R. A Microfluidic Aptamer-Based Sensor for Detection of Mercury (II) and Lead (II) Ions in Water. Preprints 2021, 2021090056 (doi: 10.20944/preprints202109.0056.v1). Huang, W.; Wu, R.; Yeh, K.; Mai, V.; Yang, R. A Microfluidic Aptamer-Based Sensor for Detection of Mercury (II) and Lead (II) Ions in Water. Preprints 2021, 2021090056 (doi: 10.20944/preprints202109.0056.v1).

Abstract

Heavy metal contaminants have serious consequences for the environment and human health. Consequently, effective methods for detecting their presence, particularly in water and food, are urgently required. Accordingly, the present study proposes a sensor for the detection of mercury Hg(II) and lead Pb(II) ions using graphene oxide (GO) as a quenching agent and aptamer solu-tion as a reagent. In the proposed device, the aptamer sequences are labeled by FAM and HEX fluorescent dyes, respectively, and are mixed with 500 ppm GO solution in a microfluidic device. The presence of Hg(II) and Pb(II) ions is then detected by measuring the change in the fluores-cence intensity of the GO/aptamer suspension as the aptamer molecules undergo fluorescence resonance energy transfer (FRET). The experimental results show that the aptamer sensors have a linear range of 10~250 nM (i.e., 2.0~50 ppb) for Hg(II) ions and 10~100 nM (i.e., 2.1~20.7 ppb) for Pb(II) ions. Furthermore, the limit of detection is around 2 ppb for both metals, which is signifi-cantly lower than the maximum limits of 6 ppb and 10 ppb prescribed by the World Health Or-ganization (WHO) for Hg(II) and Pb(II) in drinking water, respectively.

Keywords

Aptamer; Fluorescence Resonance Energy Transfer; Heavy Metal Ions; Graphene Oxide; Microfluidic Device; Sensor.

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