Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes

Marta Gomez-Perosanz
,
Tara Fiyouzi
ORCID logo ,
Miguel Fernandez-Arquero
,
John Sidney
,
Alessandro Sette
,
Ellis L. Reinherz
,
Esther M. Lafuente
ORCID logo ,
Pedro A. Reche
* ORCID logo
Version 1 : Received: 30 July 2021 / Approved: 2 August 2021 / Online: 2 August 2021 (08:43:20 CEST)

A peer-reviewed article of this Preprint also exists.

Gomez-Perosanz, M.; Fiyouzi, T.; Fernandez-Arquero, M.; Sidney, J.; Sette, A.; Reinherz, E.L.; Lafuente, E.M.; Reche, P.A. Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes. Cells 2021, 10, 2294. Gomez-Perosanz, M.; Fiyouzi, T.; Fernandez-Arquero, M.; Sidney, J.; Sette, A.; Reinherz, E.L.; Lafuente, E.M.; Reche, P.A. Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes. Cells 2021, 10, 2294.

Abstract

Human Rhinovirus (HRV) is the most common cause of upper respiratory infections and exacerbations of asthma. In this work, we selected 14 peptides (6 from HRV A and 8 from HRV C) encompassing potential CD4 T cell epitopes. Peptides were selected for being highly conserved in HRV A and C serotypes and predicted to bind to multiple HLA II molecules. We found positive T cell recall responses by IFNγ-ELISPOT assays to 8 peptides, validating 7 of them (3 from HRV A and 4 from HRV C) as CD4 T cell epitopes through intracellular cytokine staining assays. Additionally, we verified their promiscuous binding to multiple HLA II molecules by quantitative binding assays. According to their experimental HLA II binding profile, the combination of all these 7 epitopes could be presented and recognized by > 95 % of the world population. We actually determined IFNγ responses to a pool encompassing these CD4 T cell epitopes by intracellular cytokine staining, finding positive responses in 29 out of 30 donors. The CD4 T cell epitopes identified in this study could be key to monitor HRV infections and to develop peptide-based vaccines against most HRV A and C serotypes.

Keywords

Human Rhinovirus; CD4 T cell; epitope; peptide

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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