Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.)

Cobes Gatarira
,
Alžběta Němečková
ORCID logo ,
Denisa Šimoníková
ORCID logo ,
Rajneesh Paliwal
,
Asrat Asfaw
ORCID logo ,
Michael Abberton
,
Badara Gueye
,
Robert Asiedu
ORCID logo ,
Jana Čížková
,
Eva Hřibová
ORCID logo ,
Jaroslav Doležel
ORCID logo ,
David Dekoeyer
,
Victor O. Adetimirin
,
Ranjana Bhattacharjee
* ORCID logo
Version 1 : Received: 12 July 2021 / Approved: 13 July 2021 / Online: 13 July 2021 (11:29:47 CEST)

A peer-reviewed article of this Preprint also exists.

Gatarira, C.; Sládeková, L.; Němečková, A.; Šimoníková, D.; Paliwal, R.; Asfaw, A.; Abberton, M.; Badara, G.; Asiedu, R.; Čížková, J.; Hřibová, E.; Doležel, J.; DeKoeyer, D.; Adetimirin, V.; Bhattacharjee, R. Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.). Agronomy 2021, 11, 1897. Gatarira, C.; Sládeková, L.; Němečková, A.; Šimoníková, D.; Paliwal, R.; Asfaw, A.; Abberton, M.; Badara, G.; Asiedu, R.; Čížková, J.; Hřibová, E.; Doležel, J.; DeKoeyer, D.; Adetimirin, V.; Bhattacharjee, R. Cytological and Molecular Characterization for Ploidy Determination in Yams (Dioscorea spp.). Agronomy 2021, 11, 1897.

Abstract

Yam (Dioscorea spp.) is a monocotyledonous herbaceous vine plant grown in the tropics and subtropics. It is a multi-species plant with varied intra- and interspecific ploidy levels. Of the 600 species, 11 are cultivated staple supporting the livelihood of over 300 million people. The paucity of information on ploidy and the genomic constitution is a significant challenge to the crop’s genetic improvement through crossbreeding. The objective of this study was to investigate the ploidy levels of 236 accessions across six cultivated and two wild species using chromosome counting, flow cytometry and genotyping-based ploidy determination methods. Results obtained from chromosome counting and genotyping-based ploidy determination were in agreement. In majority of the accessions, chromosome counting and flow cytometry were congruent, allowing future rapid screening of ploidy levels using flow cytometry. Among cultivated accessions, 168 (71%) were diploid, 50 (21%) were triploid, and 12 (5%) were tetraploid. Two wild species included in the study were diploids. Resolution of ploidy level in yams offers opportunities for implementing successful breeding programmes through intra- and inter-specific hybridization.

Keywords

Dioscorea spp.; flow cytometry; chromosome counting; ploidy level; DArTseq

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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