Version 1
: Received: 8 July 2021 / Approved: 9 July 2021 / Online: 9 July 2021 (15:23:57 CEST)
How to cite:
Hassan, M.M.; Nam, S. Isocratic Reverse-Phase HPLC Method for Determination of Aspirin, Paracetamol, and Naproxen. Preprints2021, 2021070229. https://doi.org/10.20944/preprints202107.0229.v1
Hassan, M.M.; Nam, S. Isocratic Reverse-Phase HPLC Method for Determination of Aspirin, Paracetamol, and Naproxen. Preprints 2021, 2021070229. https://doi.org/10.20944/preprints202107.0229.v1
Hassan, M.M.; Nam, S. Isocratic Reverse-Phase HPLC Method for Determination of Aspirin, Paracetamol, and Naproxen. Preprints2021, 2021070229. https://doi.org/10.20944/preprints202107.0229.v1
APA Style
Hassan, M.M., & Nam, S. (2021). Isocratic Reverse-Phase HPLC Method for Determination of Aspirin, Paracetamol, and Naproxen. Preprints. https://doi.org/10.20944/preprints202107.0229.v1
Chicago/Turabian Style
Hassan, M.M. and Sung-Wook Nam. 2021 "Isocratic Reverse-Phase HPLC Method for Determination of Aspirin, Paracetamol, and Naproxen" Preprints. https://doi.org/10.20944/preprints202107.0229.v1
Abstract
Nonsteroidal anti-inflammatory drugs (NSAIDs), which block the activity of cyclooxygenase (COX) isoenzymes and inhibit the synthesis of prostaglandin, have been used for pain relief. We have developed a method to separate a mixture of three NSAIDs, such as aspirin, paracetamol, and naproxen, using reverse-phase high-performance liquid chromatography (RP-HPLC). An isocratic mobile phase consisting of acidic water and acetonitrile was selected to run at a low flow rate, such as 0.8 mL/min. The mixture of three NSAIDs was injected at a low volume into a C18 column that was 150 mm in length and characterized using a UV detector at 230 nm. We identified three peaks in the chromatogram indicating the three compounds. The elution time of the peaks was less than 10 minutes. To identify multiple peaks on the isocratic flow using a short column, further studies are required regarding the proposed method to generate microfluidic devices for nanoLC.
Keywords
HPLC; NSAIDs; Isocratic; Short column; Drug mixture
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
