Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region)

Version 1 : Received: 5 July 2021 / Approved: 6 July 2021 / Online: 6 July 2021 (13:28:46 CEST)

How to cite: Karabanov, D.P.; Bekker, E.I.; Pavlov, D.D.; Borovikova, E.A.; Kodukhova, Y.V.; Kotov, A.A. Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region). Preprints 2021, 2021070151 (doi: 10.20944/preprints202107.0151.v1). Karabanov, D.P.; Bekker, E.I.; Pavlov, D.D.; Borovikova, E.A.; Kodukhova, Y.V.; Kotov, A.A. Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region). Preprints 2021, 2021070151 (doi: 10.20944/preprints202107.0151.v1).

Abstract

Reliable species identification is critical for detection and monitoring of biological invasions. In this study, we propose four sets of primers for efficient amplification of several loci, including the mitochondrial cytochrome oxidase-c (COI) subunit I gene which is a basis for DNA barcoding. This set of primers gives a shorter product which can be used in high-throughput sequencing systems for metabarcoding purposes. Another mitochondrial locus encoding the large ribosomal subunit (16S) may be useful to study the population structure and as an additional source of information in the metabarcoding of communities. We propose to use a set of primers for the nuclear locus of the small ribosomal subunit (18S) as a positive control and to verify the results of the barcoding. Our proposed sets of primers demonstrate a high amplification efficiency and a high specificity both for freshwater alien and indigenous fishes. The proposed research design makes it possible to carry out extremely cheap studies on the assessment of biological diversity using genetic analysis without expensive equipment, and with the technique for conducting laboratory work and processing of the results available to any researcher. The paper also presents original data on the genetic polymorphism of all mass alien fish species in the Volga-Kama region. High efficiency of DNA identification based on our primers is shown as compared to traditional monitoring of biological invasions.

Subject Areas

biological invasions; fish; DNA; barcoding; primers; identification

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.