Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region)

Version 1 : Received: 5 July 2021 / Approved: 6 July 2021 / Online: 6 July 2021 (13:28:46 CEST)

How to cite: Karabanov, D.P.; Bekker, E.I.; Pavlov, D.D.; Borovikova, E.A.; Kodukhova, Y.V.; Kotov, A.A. Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region). Preprints 2021, 2021070151. https://doi.org/10.20944/preprints202107.0151.v1 Karabanov, D.P.; Bekker, E.I.; Pavlov, D.D.; Borovikova, E.A.; Kodukhova, Y.V.; Kotov, A.A. Universal Set of Primers for DNA Identification of Alien Fish Species in Central Russia (Volga-Kama Region). Preprints 2021, 2021070151. https://doi.org/10.20944/preprints202107.0151.v1

Abstract

Reliable species identification is critical for detection and monitoring of biological invasions. In this study, we propose four sets of primers for efficient amplification of several loci, including the mitochondrial cytochrome oxidase-c (COI) subunit I gene which is a basis for DNA barcoding. This set of primers gives a shorter product which can be used in high-throughput sequencing systems for metabarcoding purposes. Another mitochondrial locus encoding the large ribosomal subunit (16S) may be useful to study the population structure and as an additional source of information in the metabarcoding of communities. We propose to use a set of primers for the nuclear locus of the small ribosomal subunit (18S) as a positive control and to verify the results of the barcoding. Our proposed sets of primers demonstrate a high amplification efficiency and a high specificity both for freshwater alien and indigenous fishes. The proposed research design makes it possible to carry out extremely cheap studies on the assessment of biological diversity using genetic analysis without expensive equipment, and with the technique for conducting laboratory work and processing of the results available to any researcher. The paper also presents original data on the genetic polymorphism of all mass alien fish species in the Volga-Kama region. High efficiency of DNA identification based on our primers is shown as compared to traditional monitoring of biological invasions.

Keywords

biological invasions; fish; DNA; barcoding; primers; identification

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Comments (1)

Comment 1
Received: 3 February 2022
Commenter: (Click to see Publons profile: )
The commenter has declared there is no conflict of interests.
Comment: The original article:
Karabanov DP, Bekker EI, Pavlov DD, Borovikova EA, Kodukhova YV, Kotov AA. New Sets of Primers for DNA Identification of Non-Indigenous Fish Species in the Volga-Kama Basin (European Russia). Water. 2022; 14(3):437. https://doi.org/10.3390/w14030437
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