Jasmonic Acid-dependent MYC Transcription Factors Bind to a Tandem G-box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses

Marta-Marina Pérez-Alonso; Beatriz Sánchez-Parra; Paloma Ortiz-García; Estrella Santamaría; Isabel Díaz; Stephan Pollmann

doi:10.20944/preprints202107.0142.v1

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preprints.org > biology and life sciences > anatomy and physiology > doi: 10.20944/preprints202107.0142.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Jasmonic Acid-dependent MYC Transcription Factors Bind to a Tandem G-box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses

Marta-Marina Pérez-Alonso

^* ,

Beatriz Sánchez-Parra

Version 1 : Received: 2 July 2021 / Approved: 6 July 2021 / Online: 6 July 2021 (12:20:15 CEST)

A peer-reviewed article of this Preprint also exists.

Pérez-Alonso, M.-M.; Sánchez-Parra, B.; Ortiz-García, P.; Santamaría, M.E.; Díaz, I.; Pollmann, S. Jasmonic Acid-Dependent MYC Transcription Factors Bind to a Tandem G-Box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses. Int. J. Mol. Sci. 2021, 22, 9768. Pérez-Alonso, M.-M.; Sánchez-Parra, B.; Ortiz-García, P.; Santamaría, M.E.; Díaz, I.; Pollmann, S. Jasmonic Acid-Dependent MYC Transcription Factors Bind to a Tandem G-Box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses. Int. J. Mol. Sci. 2021, 22, 9768.

Abstract

The indole-3-pyruvic acid pathway is the major route for auxin biosynthesis in higher plants. Tryptophan aminotransferases (TAA1/TAR) and members of the YUCCA family of flavin-containing monooxygenases catalyze the conversion of L-tryptophan via indole-3-pyruvic acid into indole-3-acetic acid (IAA). It has been described that locally produced jasmonic acid (JA) in response to mechanical wounding, triggers de novo-formation of IAA through the induction of two YUCCA genes, YUC8 and YUC9. Here, we report the direct involvement of a small number of basic helix-loop-helix transcription factors of the MYC family in this process. We show that the JA-mediated regulation of YUC8 and YUC9 gene expression depends on the abundance of MYC2, MYC3, and MYC4. In support of this observation, seedlings of myc knockout mutants displayed a strongly reduced response to JA-mediated IAA formation. In addition, transactivation assays provided experimental evidence for the binding of the MYC transcription factors to a particular tandem G-box motif abundant in the promoter regions of YUC8 and YUC9, but not in those of the other YUCCA genes. Moreover, we clearly demonstrate that YUC8ox and YUC9ox overexpressing plants show less damage after spider mite infestation, thereby underlining a role of auxin in plant responses toward biotic stress cues.

Keywords

Arabidopsis thaliana; indole-3-acetic acid; jasmonic acid; plant hormone crosstalk; transcriptional regulation; wound response; biotic stress; growth-defense trade-off

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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