Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation

Version 1 : Received: 13 June 2021 / Approved: 14 June 2021 / Online: 14 June 2021 (16:04:21 CEST)

How to cite: Sharma, H.; Sapkota, H.P.; Aryal, S.; Dangi, N.B.; BATIHA, G.E.; Koirala, N. Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation. Preprints 2021, 2021060379 (doi: 10.20944/preprints202106.0379.v1). Sharma, H.; Sapkota, H.P.; Aryal, S.; Dangi, N.B.; BATIHA, G.E.; Koirala, N. Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation. Preprints 2021, 2021060379 (doi: 10.20944/preprints202106.0379.v1).

Abstract

UV-Visible spectroscopy method has been developed for the analysis of Thiamine in biological and pharmaceutical product, based on a chromogenic derivatizing reaction using PDAB (P- dimethyl amino benzaldehyde) reagent. A Central Composite Design (CCD) design with response surface methodology was executed for optimization of experimental conditions of drug with a smaller number of experimental trials. Three independent factors, the concentration of PDAB, the volume of PDAB reagent, and volume of HCl, were used to construct a mathematical model and study the effects of these independent factors on responses as absorbance. The colored complex exhibits a Red shift with absorption maximum λmax at 425 nm, which was selected as the analytical wavelength. The drug seems to be linear, which was established via the regression analysis from 5-30 μgmL-1, with an R2 value of 0.998.The % RSD for intraday and interday precision was < 2%, with good recovery ranging from 95.02 to 101.43% with biological and pharmaceutical samples. LOD and LOQ of the developed method were found to be 1.51 μg mL-1 and 4.57 μg mL-1.This method can be used in routine analysis of pharmaceutical products containing aromatic primary amines along with an estimation of biological samples like urine, blood, sweat, faeces.

Keywords

thiamine; PDAB reagent; UV Spectrophotometry; Design of experiment; pharmaceutical samples

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