Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Therapeutic Potential Of Human Neural Precursor Cells in Diabetic Retinopathy – Preclinical Model

Claudia Sayuri Saçaki
,
Bassam Felipe Mogharbel
ORCID logo ,
Priscila Elias Ferreira Stricker
ORCID logo ,
Dilcele Silva Moreira Dziedzic
ORCID logo ,
Ana Carolina Irioda
,
Maiara Carolina Perussolo
,
Fabiano Montiani-Ferreira
,
Juan Moreno
,
Peterson Dornbusch
,
Mario Sato
,
Naoye Shiokawa
,
Lúcia Noronha
ORCID logo ,
Seigo Nagashima
ORCID logo ,
Marianna Bacellar-Galdino
,
Célia Regina Cavichiolo Franco
,
Eltyeb Abdelwahid
,
Katherine Athayde Teixeira de Carvalho
* ORCID logo
Version 1 : Received: 5 June 2021 / Approved: 9 June 2021 / Online: 9 June 2021 (13:44:48 CEST)

A peer-reviewed article of this Preprint also exists.

Saçaki, C.S.; Mogharbel, B.F.; Stricker, P.E.F.; Dziedzic, D.S.M.; Irioda, A.C.; Perussolo, M.C.; Somma, A.T.; Montiani-Ferreira, F.; Moreno, J.C.D.; Dornbusch, P.; et al. Potential of Human Neural Precursor Cells in Diabetic Retinopathy Therapeutics – Preclinical Model. Current Eye Research 2021, 47, 450–460, doi:10.1080/02713683.2021.2002909. Saçaki, C.S.; Mogharbel, B.F.; Stricker, P.E.F.; Dziedzic, D.S.M.; Irioda, A.C.; Perussolo, M.C.; Somma, A.T.; Montiani-Ferreira, F.; Moreno, J.C.D.; Dornbusch, P.; et al. Potential of Human Neural Precursor Cells in Diabetic Retinopathy Therapeutics – Preclinical Model. Current Eye Research 2021, 47, 450–460, doi:10.1080/02713683.2021.2002909.

Abstract

This study aimed to evaluate cell therapy with human neural precursor cells (hNPCs) in diabetic retinopathy (DR) Wistar rats, induced to diabetes by intraperitoneal injection with streptozotocin. Wharton's Jelly Mesenchymal stem cells (WJ-MSCs) were isolated, expanded, and seeded onto a biopolymer substrate without growth factors to develop neurospheres to obtain the hNPCs, characterized by immunocytochemistry. The animals were divided into three groups; non-diabetic (ND) n = four; diabetic without treatment (DM) n = nine; and diabetic with cell therapy (DM + hNPCs) n = nine. After eight weeks of diabetes induction and verified DR, intravitreal injection of hNPCs (1 x 106 cel/µL) was performed in the DM + hNPCs group. Optical Coherence Tomography (OCT) and Electroretinography (ERG) evaluations were done before and after diabetes induction and after cell therapy. Eye enucleation occurred four weeks after treatment for the histopathological and immunohistochemistry analyses. In the treated group, there was the repair of the retinal structures and their arrangements. hNPCs increased the thickness of neuroretina layers, especially in the ganglion cell and photoreceptor layers. The results indicate that hNPCs reduced DR progression by a neuroprotective effect and promoted retinal repair, making them potential candidates for regeneration of the neuroretinal tissue.

Keywords

diabetic retinopathy; mesenchymal stem cells; neural precursor cells; stem cell.

Subject

Medicine and Pharmacology, Immunology and Allergy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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