Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Therapeutic Potential Of Human Neural Precursor Cells in Diabetic Retinopathy – Preclinical Model

Version 1 : Received: 5 June 2021 / Approved: 9 June 2021 / Online: 9 June 2021 (13:44:48 CEST)

How to cite: Saçaki, C.S.; Mogharbel, B.F.; Stricker, P.E.F.; Dziedzic, D.S.M.; Irioda, A.C.; Perussolo, M.C.; Montiani-Ferreira, F.; Moreno, J.; Dornbusch, P.; Sato, M.; Shiokawa, N.; Noronha, L.; Nagashima, S.; Bacellar-Galdino, M.; Franco, C.R.C.; Abdelwahid, E.; Athayde Teixeira de Carvalho, K. Therapeutic Potential Of Human Neural Precursor Cells in Diabetic Retinopathy – Preclinical Model. Preprints 2021, 2021060268 (doi: 10.20944/preprints202106.0268.v1). Saçaki, C.S.; Mogharbel, B.F.; Stricker, P.E.F.; Dziedzic, D.S.M.; Irioda, A.C.; Perussolo, M.C.; Montiani-Ferreira, F.; Moreno, J.; Dornbusch, P.; Sato, M.; Shiokawa, N.; Noronha, L.; Nagashima, S.; Bacellar-Galdino, M.; Franco, C.R.C.; Abdelwahid, E.; Athayde Teixeira de Carvalho, K. Therapeutic Potential Of Human Neural Precursor Cells in Diabetic Retinopathy – Preclinical Model. Preprints 2021, 2021060268 (doi: 10.20944/preprints202106.0268.v1).

Abstract

This study aimed to evaluate cell therapy with human neural precursor cells (hNPCs) in diabetic retinopathy (DR) Wistar rats, induced to diabetes by intraperitoneal injection with streptozotocin. Wharton's Jelly Mesenchymal stem cells (WJ-MSCs) were isolated, expanded, and seeded onto a biopolymer substrate without growth factors to develop neurospheres to obtain the hNPCs, characterized by immunocytochemistry. The animals were divided into three groups; non-diabetic (ND) n = four; diabetic without treatment (DM) n = nine; and diabetic with cell therapy (DM + hNPCs) n = nine. After eight weeks of diabetes induction and verified DR, intravitreal injection of hNPCs (1 x 106 cel/µL) was performed in the DM + hNPCs group. Optical Coherence Tomography (OCT) and Electroretinography (ERG) evaluations were done before and after diabetes induction and after cell therapy. Eye enucleation occurred four weeks after treatment for the histopathological and immunohistochemistry analyses. In the treated group, there was the repair of the retinal structures and their arrangements. hNPCs increased the thickness of neuroretina layers, especially in the ganglion cell and photoreceptor layers. The results indicate that hNPCs reduced DR progression by a neuroprotective effect and promoted retinal repair, making them potential candidates for regeneration of the neuroretinal tissue.

Keywords

diabetic retinopathy; mesenchymal stem cells; neural precursor cells; stem cell.

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.