Working Paper Article Version 1 This version is not peer-reviewed

Inducible Selectable Marker Genes to Improve Aspergillus fumigatus Genetic Manipulation

Version 1 : Received: 21 May 2021 / Approved: 24 May 2021 / Online: 24 May 2021 (13:22:44 CEST)

A peer-reviewed article of this Preprint also exists.

Baldin, C.; Kühbacher, A.; Merschak, P.; Sastré-Velásquez, L.E.; Abt, B.; Dietl, A.-M.; Haas, H.; Gsaller, F. Inducible Selectable Marker Genes to Improve Aspergillus fumigatus Genetic Manipulation. J. Fungi 2021, 7, 506. Baldin, C.; Kühbacher, A.; Merschak, P.; Sastré-Velásquez, L.E.; Abt, B.; Dietl, A.-M.; Haas, H.; Gsaller, F. Inducible Selectable Marker Genes to Improve Aspergillus fumigatus Genetic Manipulation. J. Fungi 2021, 7, 506.

Journal reference: J. Fungi 2021, 7, 506
DOI: 10.3390/jof7070506

Abstract

The hygromycin B phosphotransferase gene from Escherichia coli and the pyrithiamine resistance gene from Aspergillus oryzae are two dominant selectable marker genes widely used to genetically manipulate several fungal species. Despite the recent development of CRISPR/Cas9 and marker-free systems, in vitro molecular tools to study Aspergillus fumigatus, a saprophytic fungus causing life threatening diseases in immunocompromised hosts, still rely extensively on the use of dominant selectable markers. The limited number of drug selectable markers is already a critical aspect, but the possibility that their introduction into a microorganism could induce enhanced virulence or undesired effects on metabolic behavior, constitutes another problem. In this context, here we demonstrate that the use of ptrA in A. fumigatus leads to secretion of a compound that allows recovery of thiamine auxotrophy. In this study we developed a simple modification of the two commonly used dominant markers in which the development of resistance can be controlled by the xylose-inducible promoter PxylP from Penicillium chrysogenum. This strategy provides an easy solution to avoid undesired side effects since the marker expression can be readily silenced when not required.

Keywords

dominant selectable marker; Aspergillus fumigatus; inducible marker; hph; ptrA; xylanase promoter; thiamine; hygromycin; pyrithiamine

Subject

LIFE SCIENCES, Biochemistry

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