Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Dynamics of Bacterial Community Structure in the Rhizosphere and Root Nodule of Soybean: Impacts of Growth Stages and Varieties

Soo-In Sohn
* ,
Jae-Hyung Ahn
ORCID logo ,
Subramani Pandian
ORCID logo ,
Young-Ju Oh
,
Eun-Kyoung Shin
,
Hyeon-Jung Kang
,
Woo Suk Cho
,
Youn-Sung Cho
,
Kong-Sik Shin
Version 1 : Received: 19 May 2021 / Approved: 20 May 2021 / Online: 20 May 2021 (09:13:15 CEST)

A peer-reviewed article of this Preprint also exists.

Sohn, S.-I.; Ahn, J.-H.; Pandian, S.; Oh, Y.-J.; Shin, E.-K.; Kang, H.-J.; Cho, W.-S.; Cho, Y.-S.; Shin, K.-S. Dynamics of Bacterial Community Structure in the Rhizosphere and Root Nodule of Soybean: Impacts of Growth Stages and Varieties. Int. J. Mol. Sci. 2021, 22, 5577. Sohn, S.-I.; Ahn, J.-H.; Pandian, S.; Oh, Y.-J.; Shin, E.-K.; Kang, H.-J.; Cho, W.-S.; Cho, Y.-S.; Shin, K.-S. Dynamics of Bacterial Community Structure in the Rhizosphere and Root Nodule of Soybean: Impacts of Growth Stages and Varieties. Int. J. Mol. Sci. 2021, 22, 5577.

Abstract

Bacterial communities in rhizosphere and root nodules have significant contributions to the growth and productivity of the soybean (Glycine max L.). In this report, we analyzed the physiological properties and dynamics of bacterial community structure in rhizosphere and root nodules at different growth stages using BioLog EcoPlate and high-throughput sequencing technology, respectively. The BioLog assay found that the metabolic capability of rhizosphere is in increasing trend in the growth of soybeans as compared to the bulk soil. As a result of the Illumina sequencing analysis, the microbial community structure of rhizosphere and root nodules was found to be influenced by the variety and growth stage of the soybean. At the phylum level, Actinobacteria were the most abundant in rhizosphere at all growth stages, followed by Alphaproteobacteria and Acidobacteria and the phylum Bacteroidetes showed the greatest change. But, in the root nodules Alphaproteobacteria were dominant. The results of the OTU analysis exhibited the dominance of Bradyrhizobium during the entire stage of growth, but the ratio of non-rhizobial bacteria showed an increasing trend as the soybean growth progressed. These findings revealed that bacterial community in the rhizosphere and root nodules changed according to both the variety and growth stages of soybean in the field.

Keywords

Bacteria; community composition; growth stage; rhizosphere; root nodule; soybean; 16S rRNA gene

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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