Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biofilm Inhibition and Antimicrobial Properties of Silver Ion-Exchanged Zeolite a against Vibrio spp Marine Pathogens

Version 1 : Received: 3 April 2021 / Approved: 5 April 2021 / Online: 5 April 2021 (14:25:07 CEST)

A peer-reviewed article of this Preprint also exists.

Amin, Z.; Waly, N.A.; Arshad, S.E. Biofilm Inhibition and Antimicrobial Properties of Silver-Ion-Exchanged Zeolite A against Vibrio spp Marine Pathogens. Appl. Sci. 2021, 11, 5496. Amin, Z.; Waly, N.A.; Arshad, S.E. Biofilm Inhibition and Antimicrobial Properties of Silver-Ion-Exchanged Zeolite A against Vibrio spp Marine Pathogens. Appl. Sci. 2021, 11, 5496.

Journal reference: Appl. Sci. 2021, 11, 5496
DOI: 10.3390/app11125496

Abstract

A challenging problem in the aquaculture industry is bacterial disease outbreaks which results in the global reduction of fish supply and foodborne outbreaks. Biofilms in marine pathogens protect against antimicrobial treatment and host immune defence. Zeolites are minerals of volcanic origin made from crystalline aluminosilicates which are useful in agriculture and in environmental management. In this study, silver ion-exchanged zeolite A of four concentrations; 0.25M (AgZ1), 0.50M (AgZ2), 1.00M (AgZ3)and 1.50M (AgZ4) were investigated for biofilm inhibition and antimicrobial properties against two predominant marine pathogens V. campbelli and V. parahemolyticus by employing the Minimum Inhibitory Concentration(MIC), Crystal Violet Biofilm Quantification assays as well as Scanning Electron Microscopy. In the first instance, all zeolite samples AgZ1-AgZ4 showed antimicrobial activity for both pathogens. For V. campbellii AgZ4 exhibited the highest MIC at 125.00 µg/ml while for V. parahaemolyticus the highest MIC was observed for AgZ3 at 62.50 µg/ml. At sublethal concentration, biofilm inhibition of V. campbelli and V. parahemolyticus by AgZ4 were observed at 60.2% and 77.3% inhibition respectively. Scanning electron microscopy exhibited profound structural alteration of the biofilm matrix by AgZ4. This is the first known study that highlights the potential application of ion-exchanged Zeolite A against marine pathogens and their biofilms.

Keywords

Biofilms; Zeolite A; Vibrio sp; Antimicrobials

Subject

LIFE SCIENCES, Biochemistry

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