Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide-Nucleic Acid Sequences

Isabela Dragomir
,
Alina Asandei
ORCID logo ,
Irina Schiopu
ORCID logo ,
Ioana Bucataru
,
Loredana Mereuta
,
Tudor Luchian
* ORCID logo
Version 1 : Received: 20 March 2021 / Approved: 22 March 2021 / Online: 22 March 2021 (11:08:30 CET)

A peer-reviewed article of this Preprint also exists.

Dragomir, I.S.; Asandei, A.; Schiopu, I.; Bucataru, I.C.; Mereuta, L.; Luchian, T. The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide Nucleic Acid Sequences. Polymers 2021, 13, 1210. Dragomir, I.S.; Asandei, A.; Schiopu, I.; Bucataru, I.C.; Mereuta, L.; Luchian, T. The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide Nucleic Acid Sequences. Polymers 2021, 13, 1210.

Abstract

Quantum leaps advances in the single-molecule investigative science have been made possible over the past decades through the implication of nanopores, as versatile components on dedicated biosensors. Here, we employed the nanopore-tweezing technique to capture amino acid-functionalized, peptide-nucleic acids (PNA) with -hemolysin-based nanopores, and correlate the ensuing stochastic fluctuations of the ionic current through the nanopore with the composition and order of bases in the PNAs primary structure. We demonstrate that while the system enables detection of distinct bases on homopolymeric PNA or triplet bases on heteropolymeric strands, it also reveals rich insights into the conformational dynamics of the entrapped PNA within the nanopore, relevant for perfecting the recognition capability single-molecule sequencing.

Keywords

nanopore tweezer; peptide nucleic acid; sequencing; single molecule recordings

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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