Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide-Nucleic Acid Sequences

Version 1 : Received: 20 March 2021 / Approved: 22 March 2021 / Online: 22 March 2021 (11:08:30 CET)

A peer-reviewed article of this Preprint also exists.

Dragomir, I.S.; Asandei, A.; Schiopu, I.; Bucataru, I.C.; Mereuta, L.; Luchian, T. The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide Nucleic Acid Sequences. Polymers 2021, 13, 1210. Dragomir, I.S.; Asandei, A.; Schiopu, I.; Bucataru, I.C.; Mereuta, L.; Luchian, T. The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide Nucleic Acid Sequences. Polymers 2021, 13, 1210.

Journal reference: Polymers 2021, 13, 1210
DOI: 10.3390/polym13081210

Abstract

Quantum leaps advances in the single-molecule investigative science have been made possible over the past decades through the implication of nanopores, as versatile components on dedicated biosensors. Here, we employed the nanopore-tweezing technique to capture amino acid-functionalized, peptide-nucleic acids (PNA) with -hemolysin-based nanopores, and correlate the ensuing stochastic fluctuations of the ionic current through the nanopore with the composition and order of bases in the PNAs primary structure. We demonstrate that while the system enables detection of distinct bases on homopolymeric PNA or triplet bases on heteropolymeric strands, it also reveals rich insights into the conformational dynamics of the entrapped PNA within the nanopore, relevant for perfecting the recognition capability single-molecule sequencing.

Subject Areas

nanopore tweezer; peptide nucleic acid; sequencing; single molecule recordings

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