Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Tracheal Aspirate miRNA Signatures in Preterm Infants with Severe Bronchopulmonary Dysplasia

Version 1 : Received: 4 February 2021 / Approved: 5 February 2021 / Online: 5 February 2021 (14:08:26 CET)

A peer-reviewed article of this Preprint also exists.

Siddaiah, R.; Oji-Mmuo, C.N.; Montes, D.T.; Fuentes, N.; Spear, D.; Donnelly, A.; Silveyra, P. MicroRNA Signatures Associated with Bronchopulmonary Dysplasia Severity in Tracheal Aspirates of Preterm Infants. Biomedicines 2021, 9, 257. Siddaiah, R.; Oji-Mmuo, C.N.; Montes, D.T.; Fuentes, N.; Spear, D.; Donnelly, A.; Silveyra, P. MicroRNA Signatures Associated with Bronchopulmonary Dysplasia Severity in Tracheal Aspirates of Preterm Infants. Biomedicines 2021, 9, 257.

Journal reference: Biomedicines 2021, 9, 257
DOI: 10.3390/biomedicines9030257

Abstract

Bronchopulmonary dysplasia (BPD) is a form of chronic lung disease that develops in neonates as a consequence of preterm birth and arrested fetal lung development. The incidence of BPD remains on the rise, as a result of increasing survival of extremely preterm infants. Severe BPD contributes to significant health care costs and is associated with prolonged hospitalizations, respiratory infections, and neurodevelopmental deficits. In this study, we aimed to detect novel biomarkers of severe BPD. We collected tracheal aspirates (TA) from preterm babies with mild/moderate (n = 8) and severe (n = 17) BPD, and we profiled the expression of 1048 miRNAs using a PCR array. Associations with biological pathways were determined with the Ingenuity Pathway Analysis (IPA) software. We found 31 miRNAs differentially expressed between the two disease groups (2-fold change, FDR < 0.05). Of these, 4 miRNAs displayed significantly higher expression levels, and 27 miRNAs had significantly lower expression levels in the severe BPD vs. the mild/moderate BPD group. IPA identified cell signaling and inflammation pathways associated with miRNA signatures. We conclude that TAs of extreme premature infants contain miRNA signatures associated with severe BPD. These signatures may serve as biomarkers of disease severity in infants with BPD.

Keywords

Bronchopulmonary Dysplasia; Prematurity; MiRNA; Biomarkers; Tracheal Aspirates

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