Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Detection and Identification of Babesia spp., and Trypanosoma spp. in One-humped Camel (Camelus dromedarius) in Halayeb and Shalateen, Egypt

Shimaa Abd El-Salam El-Sayed
,
Mohamed El-Adl
,
Mayar Ali
,
Mostafa Al-Araby
,
Mosaab Omar
ORCID logo ,
Mohamed El-Beskawy
,
Shimaa sorour
,
Mohammad Al-Wabel
,
Mohamed Rizk
* ORCID logo ,
Magdy Elgioushy
Version 1 : Received: 29 September 2020 / Approved: 15 October 2020 / Online: 15 October 2020 (16:27:16 CEST)

How to cite: El-Sayed, S.A.E.; El-Adl, M.; Ali, M.; Al-Araby, M.; Omar, M.; El-Beskawy, M.; sorour, S.; Al-Wabel, M.; Rizk, M.; Elgioushy, M. Molecular Detection and Identification of Babesia spp., and Trypanosoma spp. in One-humped Camel (Camelus dromedarius) in Halayeb and Shalateen, Egypt. Preprints 2020, 2020100334. https://doi.org/10.20944/preprints202010.0334.v1 El-Sayed, S.A.E.; El-Adl, M.; Ali, M.; Al-Araby, M.; Omar, M.; El-Beskawy, M.; sorour, S.; Al-Wabel, M.; Rizk, M.; Elgioushy, M. Molecular Detection and Identification of Babesia spp., and Trypanosoma spp. in One-humped Camel (Camelus dromedarius) in Halayeb and Shalateen, Egypt. Preprints 2020, 2020100334. https://doi.org/10.20944/preprints202010.0334.v1

Abstract

Phylogenetic analysis of blood parasite infections including Babesia (B.) bovis, Babesia microti and Trypanosoma (T.) spp. in one-humped camel (Camelus dromedarius) (n= 142) breeds in Halayeb and Shalateen, in Upper Egypt were performed in the current study. Polymerase chain reaction (PCR) assays targeting the Rhoptry Associated Protein-1 (RAP-1), Babesia microti small subunit rRNA (ss-rRNA) and internal transcribed spacer 1 (ITS1) genes were used to detect the prevalence of B. bovis, B. microti and Trypanosoma spp. in camels, respectively. Nested PCR assays were used for the detection of Babesia spp. (B. bovis and B. microti). While, KIN-multi species PCR reaction was employed to detect and identify trypanosome DNA in camels. B. microti was detected in (17/142) with infection rate (11.97 %). Sequencing and phylogenetic analyses revealed that B. microti detected in camel was closely related to the German strain in rats and voles in France. B. bovis was also detected in (4/142) with infection rate (2.81%). The sequence and phylogenetic analyses revealed that the isolated B. bovis was closely related to strains isolated from Argentine, USA and Brazil. Moreover, T. evansi was detected in (8/142) with infection rate (5.63%). Sequence and phylogenetic analyses revealed that isolated T. evansi was closely related to T. theileri that was detected from cattle in Brazil. This study provides the first evidence of B. microti in camel in Egypt and highlights the possible role of one-humped camels in maintaining the enzootic cycle of Babesia transmission in Egypt.

Keywords

Babesia spp.; Trypanosoma spp.; Camel; Egypt; Epidemiology

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.