Working Paper Article Version 2 This version is not peer-reviewed

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Version 1 : Received: 12 October 2020 / Approved: 14 October 2020 / Online: 14 October 2020 (12:40:15 CEST)
Version 2 : Received: 29 October 2020 / Approved: 30 October 2020 / Online: 30 October 2020 (09:02:45 CET)

A peer-reviewed article of this Preprint also exists.

Carvalho, S.; Carneiro, I.; Henrique, R.; Tuchin, V.; Oliveira, L. Lipofuscin-Type Pigment as a Marker of Colorectal Cancer. Electronics 2020, 9, 1805. Carvalho, S.; Carneiro, I.; Henrique, R.; Tuchin, V.; Oliveira, L. Lipofuscin-Type Pigment as a Marker of Colorectal Cancer. Electronics 2020, 9, 1805.


The study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method and with the exception of the reduced scattering coefficient, which was obtained by traditional simulation methods, all the other optical properties were calculated in a simple and fast manner for human and pathological colorectal tissues. The obtained results are in good agreement with previous published data, both in magnitude and in wavelength dependence. Since this method is based on spectral measurements and not on discrete-wavelength experimental data, the calculated optical properties contain spectral signatures that correspond to major tissue chromophores such as DNA and hemoglobin. Analysis of the absorption bands of hemoglobin in the wavelength dependence of the absorption spectra of normal and pathological colorectal mucosa allowed to identify differentiated accumulation of a pigment in these tissues. The increased content of this pigment in the pathological mucosa may be used for the future development of noninvasive diagnostic methods for colorectal cancer detection.


optical properties of tissues; tissue spectroscopy; differentiated pigment content; colorectal cancer; optical cancer detection; absorption coefficient; scattering coefficient; scattering anisotropy; light penetration depth


Biology and Life Sciences, Biochemistry and Molecular Biology

Comments (1)

Comment 1
Received: 30 October 2020
Commenter: Luis Oliveira
Commenter's Conflict of Interests: Author
Comment: Figures 4 to 12 were rearranged.
Equation 19 was replaced to match the reported wavelength dependence of the absoption of lipofuscin. Consequently data in graphs of Figure 13 were recalculated.
Some small corrections were made along the text to improve English.
A new reference, 38 in the list was added.
+ Respond to this comment

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 1
Metrics 0

Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.