Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Whey Proteins Characterization, Free Amino Acids Profile and Antimicrobial Study of Some Dairy Drinks based on Milk Serum

Doina Prodan
ORCID logo ,
Miuța Filip
* ORCID logo ,
Mihaela Vlassa
,
Marioara Moldovan
,
Rahela Carpa
ORCID logo ,
Viorica Tarmure
,
and Andreea Simona Popa
Version 1 : Received: 23 September 2020 / Approved: 25 September 2020 / Online: 25 September 2020 (11:45:09 CEST)

A peer-reviewed article of this Preprint also exists.

PRODAN, D.; FILIP, M.; VLASSA, M.; MOLDOVAN, M.; CARPA, R. Chromatographic Profile of Major Whey Proteins in Some Dairy Beverages Based on Milk Serum. Turkish Journal of Chemistry 2022, 46, 1999–2009, doi:10.55730/1300-0527.3497. PRODAN, D.; FILIP, M.; VLASSA, M.; MOLDOVAN, M.; CARPA, R. Chromatographic Profile of Major Whey Proteins in Some Dairy Beverages Based on Milk Serum. Turkish Journal of Chemistry 2022, 46, 1999–2009, doi:10.55730/1300-0527.3497.

Abstract

The aim of this study was characterization of some dairy drinks based on Milk Serum regarding major whey proteins (WP) and free amino acids (FAAs) using reversed phase high performance liquid chromatographic (RP-HPLC) methods. The studied WP, -lactalbumin (-La), bovine serum albumin (BSA), -lactoglobulin A (-Lg A) and -lactoglobulin B (-Lg B) were separated on Aeris XB-C18 column at 214 nm detection. The RP-HPLC method was validated by selectivity, linearity (R2 ≥0.99), sensitivity (LOQ, 1.35–10.08 µg mL−1), accuracy (recovery 96.79-103.07%) and precision (% RSD ≤ 4.13%). The total studied WP in studied dairy drinks varied between 1.42 and 3.047 g·L-1. The chromatographic profile of FAAs (aspartic acid, glutamic acid, serine, histidine, arginine, glycine, threonine, alanine, tyrosine, cysteine, tryptophan, methionine, valine, phenylalanine, isoleucine, leucine and lysine) was determined in lyophilized concentrate of Milk Serum by RP-HPLC using pre-column derivatization reaction with orthophthalaldehyde (OPA). The total studied FAAs in studied samples varied between 1.103 and 1.119 mg·g-1. Moreover, the Milk Serum showed bacteriostatic activity against two bacterial strains Escherichia coli and Staphylococcus aureus. The obtained results confirm that dairy drinks based on the Milk Serum constitutes a valuable sources of bioactive components with benefits for human healthy nutrition.

Keywords

Milk Serum; whey proteins; RP-HPLC-UV; free amino acids; RP-HPLC-FLD; antimicrobial study

Subject

Chemistry and Materials Science, Analytical Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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