Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Whey Proteins Characterization, Free Amino Acids Profile and Antimicrobial Study of Some Dairy Drinks based on Milk Serum

Version 1 : Received: 23 September 2020 / Approved: 25 September 2020 / Online: 25 September 2020 (11:45:09 CEST)

How to cite: Prodan, D.; Filip, M.; Vlassa, M.; Moldovan, M.; Carpa, R.; Tarmure, V.; Popa, A.A.S. Whey Proteins Characterization, Free Amino Acids Profile and Antimicrobial Study of Some Dairy Drinks based on Milk Serum. Preprints 2020, 2020090607 (doi: 10.20944/preprints202009.0607.v1). Prodan, D.; Filip, M.; Vlassa, M.; Moldovan, M.; Carpa, R.; Tarmure, V.; Popa, A.A.S. Whey Proteins Characterization, Free Amino Acids Profile and Antimicrobial Study of Some Dairy Drinks based on Milk Serum. Preprints 2020, 2020090607 (doi: 10.20944/preprints202009.0607.v1).

Abstract

The aim of this study was characterization of some dairy drinks based on Milk Serum regarding major whey proteins (WP) and free amino acids (FAAs) using reversed phase high performance liquid chromatographic (RP-HPLC) methods. The studied WP, -lactalbumin (-La), bovine serum albumin (BSA), -lactoglobulin A (-Lg A) and -lactoglobulin B (-Lg B) were separated on Aeris XB-C18 column at 214 nm detection. The RP-HPLC method was validated by selectivity, linearity (R2 ≥0.99), sensitivity (LOQ, 1.35–10.08 µg mL−1), accuracy (recovery 96.79-103.07%) and precision (% RSD ≤ 4.13%). The total studied WP in studied dairy drinks varied between 1.42 and 3.047 g·L-1. The chromatographic profile of FAAs (aspartic acid, glutamic acid, serine, histidine, arginine, glycine, threonine, alanine, tyrosine, cysteine, tryptophan, methionine, valine, phenylalanine, isoleucine, leucine and lysine) was determined in lyophilized concentrate of Milk Serum by RP-HPLC using pre-column derivatization reaction with orthophthalaldehyde (OPA). The total studied FAAs in studied samples varied between 1.103 and 1.119 mg·g-1. Moreover, the Milk Serum showed bacteriostatic activity against two bacterial strains Escherichia coli and Staphylococcus aureus. The obtained results confirm that dairy drinks based on the Milk Serum constitutes a valuable sources of bioactive components with benefits for human healthy nutrition.

Subject Areas

Milk Serum; whey proteins; RP-HPLC-UV; free amino acids; RP-HPLC-FLD; antimicrobial study

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