Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Impact of Eukaryotic Enteric Pathogens on Malian Children’s Gut Mycobiota

Aly Kodio
,
Estelle Menu
,
Safiatou Doumbo
,
Drissa Coulibaly
ORCID logo ,
Abdoulaye Kassoum Koné
,
Salimata Konaté
,
Mamadou Lamine Tall
,
Ogobara K. Doumbo
,
Didier Raoult
,
Mahamadou Aly Thera
,
Stéphane Ranque
*
Version 1 : Received: 23 September 2020 / Approved: 24 September 2020 / Online: 24 September 2020 (12:24:53 CEST)

How to cite: Kodio, A.; Menu, E.; Doumbo, S.; Coulibaly, D.; Koné, A. K.; Konaté, S.; Tall, M. L.; Doumbo, O. K.; Raoult, D.; Thera, M. A.; Ranque, S. Impact of Eukaryotic Enteric Pathogens on Malian Children’s Gut Mycobiota. Preprints 2020, 2020090584. https://doi.org/10.20944/preprints202009.0584.v1 Kodio, A.; Menu, E.; Doumbo, S.; Coulibaly, D.; Koné, A. K.; Konaté, S.; Tall, M. L.; Doumbo, O. K.; Raoult, D.; Thera, M. A.; Ranque, S. Impact of Eukaryotic Enteric Pathogens on Malian Children’s Gut Mycobiota. Preprints 2020, 2020090584. https://doi.org/10.20944/preprints202009.0584.v1

Abstract

Eukaryotic enteric pathogens (EEP) are a public health issue in tropical areas. Yet, their interactions with the gut mycobiota remain poorly understood. We conducted a cross-sectional study in Malian children to analyze the impact of EEP on the gut fungal community. EEP were assessed by qPCR and the gut mycobiota was characterized by ITS1-2 metabarcoding in stool samples collected from 296 children. The 100 controls, in whom no EEP was detected, were compared to: a) 196 children with ≥1 EEP; b) 91 with only Blastocystis; c) 35 with only Giardia intestinalis; and d) 12 with another (<1% each) EPP. The gut fungal community structure was homogenous in each children’s group. Linear size-effect discriminant analysis highlighted five relatively more abundant species, including Fusarium longipes and Penicillium caseifulvum, in children with ≥1 EEP, whereas 28, including Aspergillus sydowii and Microdochium colombiense were more abundant in controls. Fusarium, Pyxidiophora, and Stereum abundance was higher in Blastocystis-infected children, whereas Ogataea and Allocryptovalsa were more abundant in controls. Sordariales and Mortierellales abundance was higher in Giardia intestinalis-infected children, whereas Agaricales and Capnodiales abundance was higher in controls. In conclusion, EEP do not significantly alter the gut fungal community structure, and further studies are warranted to confirm our findings that particular taxa are associated with susceptibility or resistance to specific EEP.

Keywords

fungal community; gut mycobiota; diversity; ITS metabarcoding; intestinal parasites; children; Mali

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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