Zhang, R.; Li, C.; Yu, M.; Huang, X.; Zhang, M.; Liu, S.; Pan, S.; Xue, W.; Wang, C.; Mao, C.; Zhang, H.; Fan, G. Chromosome-Level Genome Assembly of The Humpback Puffer, Tetraodon palembangensis. Preprints2020, 2020080694
APA Style
Zhang, R., Li, C., Yu, M., Huang, X., Zhang, M., Liu, S., Pan, S., Xue, W., Wang, C., Mao, C., Zhang, H., & Fan, G. (2020). Chromosome-Level Genome Assembly of The Humpback Puffer, <em>Tetraodon palembangensis</em>. Preprints. https://doi.org/
Chicago/Turabian Style
Zhang, R., He Zhang and Guangyi Fan. 2020 "Chromosome-Level Genome Assembly of The Humpback Puffer, <em>Tetraodon palembangensis</em>" Preprints. https://doi.org/
Abstract
The humpback puffer, Tetraodon palembangensis, also known as Pao palembangensis, is a species of poisonous freshwater pufferfish mainly distributed in Southeast Asia (Thailand, Laos, Malaysia and Indonesia). Despite interesting biological features, such as its very inactive nature, tetrodotoxin production and body expansion mechanisms, molecular research on the humpback puffer is still rare because of the lack of a high-quality reference genome. Here, we reported a first chromosome-level genome assembly of an adult humpback puffer, of which the genome size is 362 Mb with ~1.78 Mb contig N50 and ~15.8 Mb scaffold N50s. Based on the genome, ~61.5Mb (18.11%) repeat sequences were also identified, and totally 19,925 genes were annotated, 99.20% of which could be predicted with function using protein-coding function databases. Finally, a phylogenetic tree was constructed with single-copy gene families from ten teleost fishes. The humpback puffer genome will be a valuable genomic resource to illustrate possible mechanisms of tetrodotoxin synthesis and tolerance, providing clues for future detailed studies of biological toxins.
Keywords
tetraodon palembangensis; chromosome-level genome; genomic annotation; gene family
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
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