Working Paper Article Version 1 This version is not peer-reviewed

Chromosome-Level Genome Assembly of The Humpback Puffer, Tetraodon palembangensis

Version 1 : Received: 27 August 2020 / Approved: 31 August 2020 / Online: 31 August 2020 (04:28:47 CEST)

How to cite: Zhang, R.; Li, C.; Yu, M.; Huang, X.; Zhang, M.; Liu, S.; Pan, S.; Xue, W.; Wang, C.; Mao, C.; Zhang, H.; Fan, G. Chromosome-Level Genome Assembly of The Humpback Puffer, Tetraodon palembangensis. Preprints 2020, 2020080694 Zhang, R.; Li, C.; Yu, M.; Huang, X.; Zhang, M.; Liu, S.; Pan, S.; Xue, W.; Wang, C.; Mao, C.; Zhang, H.; Fan, G. Chromosome-Level Genome Assembly of The Humpback Puffer, Tetraodon palembangensis. Preprints 2020, 2020080694

Abstract

The humpback puffer, Tetraodon palembangensis, also known as Pao palembangensis, is a species of poisonous freshwater pufferfish mainly distributed in Southeast Asia (Thailand, Laos, Malaysia and Indonesia). Despite interesting biological features, such as its very inactive nature, tetrodotoxin production and body expansion mechanisms, molecular research on the humpback puffer is still rare because of the lack of a high-quality reference genome. Here, we reported a first chromosome-level genome assembly of an adult humpback puffer, of which the genome size is 362 Mb with ~1.78 Mb contig N50 and ~15.8 Mb scaffold N50s. Based on the genome, ~61.5Mb (18.11%) repeat sequences were also identified, and totally 19,925 genes were annotated, 99.20% of which could be predicted with function using protein-coding function databases. Finally, a phylogenetic tree was constructed with single-copy gene families from ten teleost fishes. The humpback puffer genome will be a valuable genomic resource to illustrate possible mechanisms of tetrodotoxin synthesis and tolerance, providing clues for future detailed studies of biological toxins.

Subject Areas

tetraodon palembangensis; chromosome-level genome; genomic annotation; gene family

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