Version 1
: Received: 24 August 2020 / Approved: 26 August 2020 / Online: 26 August 2020 (09:16:38 CEST)
How to cite:
Son, E.; Choi, H. J.; Kim, S.-H.; Jang, D.-G.; Cha, J.; Choi, J. J.; Kim, M. R.; Kim, D.-S. Anti-diabetic Effects of Wild Soybean Glycine Soja Seed Extract on Type 2 Diabetic Mice and Human Hepatocytes induced Insulin Resistance. Preprints2020, 2020080572. https://doi.org/10.20944/preprints202008.0572.v1
Son, E.; Choi, H. J.; Kim, S.-H.; Jang, D.-G.; Cha, J.; Choi, J. J.; Kim, M. R.; Kim, D.-S. Anti-diabetic Effects of Wild Soybean Glycine Soja Seed Extract on Type 2 Diabetic Mice and Human Hepatocytes induced Insulin Resistance. Preprints 2020, 2020080572. https://doi.org/10.20944/preprints202008.0572.v1
Son, E.; Choi, H. J.; Kim, S.-H.; Jang, D.-G.; Cha, J.; Choi, J. J.; Kim, M. R.; Kim, D.-S. Anti-diabetic Effects of Wild Soybean Glycine Soja Seed Extract on Type 2 Diabetic Mice and Human Hepatocytes induced Insulin Resistance. Preprints2020, 2020080572. https://doi.org/10.20944/preprints202008.0572.v1
APA Style
Son, E., Choi, H. J., Kim, S. H., Jang, D. G., Cha, J., Choi, J. J., Kim, M. R., & Kim, D. S. (2020). Anti-diabetic Effects of Wild Soybean <em>Glycine Soja</em> Seed Extract on Type 2 Diabetic Mice and Human Hepatocytes induced Insulin Resistance. Preprints. https://doi.org/10.20944/preprints202008.0572.v1
Chicago/Turabian Style
Son, E., Mee Ree Kim and Dong-Seon Kim. 2020 "Anti-diabetic Effects of Wild Soybean <em>Glycine Soja</em> Seed Extract on Type 2 Diabetic Mice and Human Hepatocytes induced Insulin Resistance" Preprints. https://doi.org/10.20944/preprints202008.0572.v1
Abstract
Anti-diabetic effects of Glycine soja seed extract (GS) on Type 2 diabetes mellitus mouse model and human hepatocytes induced insulin resistance were investigated. 3 weeks old db/db mice were divided into 5 groups (n = 6) including two control groups and 3 GS treated groups with different doses. Oral administration of GS for 6 weeks to diabetic db/db mice reduced blood glucose level significantly in a dose dependent manner by 44.7% (300 mg/kg/day), 30.9% (150 mg/kg/day) and 21.1% (75 mg/kg/day). GS treatment also lowered significantly plasma level of HbA1c, insulin, IGF-1 and leptin, and increased that of adiponectin. GS treatment activated AMPK, and down-regulated GLUT2 in liver tissues of mice while up-regulated GLUT4 in muscle tissues of mice. In in vitro study with insulin resistance induced human hepatocyte, GS treatment increased glucose uptake and increased the activities of Akt and PPAR-γ in response to insulin. Treatment of GS appears to reduce blood glucose level by regulating energy metabolism positively through various metabolic pathways and reducing insulin resistance in Type 2 diabetes mellitus.
Keywords
glycine soja seed; type 2 diabetes mellitus; antidiabetic; AMPK; Akt; PPAR-γ
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
