Working Paper Article Version 1 This version is not peer-reviewed

Anti-Diabetic Effects of Wild Soybean Glycine Soja Seed Extract on Type 2 Diabetic Mice and Human Hepatocytes Induced Insulin Resistance

Version 1 : Received: 24 August 2020 / Approved: 25 August 2020 / Online: 25 August 2020 (11:47:20 CEST)

How to cite: Son, E.; Choi, H.J.; Kim, S.; Jang, D.; Cha, J.; Choi, J.J.; Kim, M.R.; Kim, D. Anti-Diabetic Effects of Wild Soybean Glycine Soja Seed Extract on Type 2 Diabetic Mice and Human Hepatocytes Induced Insulin Resistance. Preprints 2020, 2020080552 Son, E.; Choi, H.J.; Kim, S.; Jang, D.; Cha, J.; Choi, J.J.; Kim, M.R.; Kim, D. Anti-Diabetic Effects of Wild Soybean Glycine Soja Seed Extract on Type 2 Diabetic Mice and Human Hepatocytes Induced Insulin Resistance. Preprints 2020, 2020080552

Abstract

Anti-diabetic effects of Glycine soja seed extract (GS) on Type 2 diabetes mellitus mouse model and human hepatocytes induced insulin resistance were investigated. 3 weeks old db/db mice were divided into 5 groups (n = 6) including two control groups and 3 GS treated groups with different doses. Oral administration of GS for 6 weeks to diabetic db/db mice reduced blood glucose level significantly in a dose dependent manner by 44.7% (300 mg/kg/day), 30.9% (150 mg/kg/day) and 21.1% (75 mg/kg/day). GS treatment also lowered significantly plasma level of HbA1c, insulin, IGF-1 and leptin, and increased that of adiponectin. GS treatment activated AMPK, and down-regulated GLUT2 in liver tissues of mice while up-regulated GLUT4 in muscle tissues of mice. In in vitro study with insulin resistance induced human hepatocyte, GS treatment increased glucose uptake and increased the activities of Akt and PPAR-γ in response to insulin. Treatment of GS appears to reduce blood glucose level by regulating energy metabolism positively through various metabolic pathways and reducing insulin resistance in Type 2 diabetes mellitus.

Subject Areas

Glycine soja seed; Type 2 diabetes mellitus; Antidiabetic; AMPK; Akt; PPAR-γ

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