Submitted:
07 August 2020
Posted:
08 August 2020
Read the latest preprint version here
Preprints on COVID-19 and SARS-CoV-2
Abstract
RaTG13 is the next relative of SARS-CoV-2 derived from bat feces. The Illumina based NGS sequence of RaTG13 MN996532.1 was deposited on 27th Jan 2020 and the raw data, a little later on 13th Feb 2020 https://www.ncbi.nlm.nih.gov/sra/SRX7724752[accn]. The fecal swab sample shows abnormally high reads from eukaryotes which includes not only bats but other animals, as per the NCBI site. Also, comparison of the fecal swab to other bat fecal swabs deposited by the same group on the same date indicates that the fecal swab from which RaTG13 sequence was derived looked abnormal. The proportion of bacteria in this RNA Seq project was only 0.7% in contrast to 70-90% abundance in other fecal swabs from bats. Also, the amplicon sequencing done on the same sample showed large number of gaps and inconsistencies. This poses a question on the authenticity of the RaTG13 sequence also.
Keywords:
RaTG13
; SARS-COV-2
; Illumina sequencing
; amplicon sequencing
; NGS
; fecal swab
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