Development Perspective of Bioelectrocatalysis-based Biosensors

Taiki Adachi; Yuki Kitazumi; Osamu Shirai; Kenji Kano

doi:10.20944/preprints202007.0459.v1

Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 19 July 2020 / Approved: 20 July 2020 / Online: 20 July 2020 (08:16:47 CEST)

Bioelectrocatalysis provides the intrinsic catalytic-functions of redox enzymes to non-specific electrode reactions and is the most important and basic concept for biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multi-analyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.

current-potential curve; multi-enzymatic cascades; multi-analyte detection; mass-transfer-controlled amperometric response; potentiometric coulometry

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Development Perspective of Bioelectrocatalysis-based Biosensors

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