Working Paper Article Version 1 This version is not peer-reviewed

Significant Decreased Expressions of CaN, VEGF, SLC39A6 and SFRP1 in MDA-MB-231 Xenograft Breast Tumour Mice Treated with Moringa oleifera Leaves and Seed Residue (MOLSr) Extracts

Version 1 : Received: 18 July 2020 / Approved: 19 July 2020 / Online: 19 July 2020 (21:09:28 CEST)

How to cite: Lim, W.F.; Miuhammad Yusof, M.I.; Teh, L.K.; Salleh, M.Z. Significant Decreased Expressions of CaN, VEGF, SLC39A6 and SFRP1 in MDA-MB-231 Xenograft Breast Tumour Mice Treated with Moringa oleifera Leaves and Seed Residue (MOLSr) Extracts. Preprints 2020, 2020070447 Lim, W.F.; Miuhammad Yusof, M.I.; Teh, L.K.; Salleh, M.Z. Significant Decreased Expressions of CaN, VEGF, SLC39A6 and SFRP1 in MDA-MB-231 Xenograft Breast Tumour Mice Treated with Moringa oleifera Leaves and Seed Residue (MOLSr) Extracts. Preprints 2020, 2020070447

Abstract

Moringa oleifera is a miracle plant with many nutritional and medicinal properties. Chemopreventive value of the combined mixture of moringa leaves and seed residues (MOLSr) at different ratios (M1S9, M1S1 and M9S1) were investigated. MOLSr extracts were subjected to phytochemical screening, antioxidant assays, metabolite profiling and cytotoxic activity on various human cancer cell lines including primary mammary epithelial cells (PMEC). The MOLSr ratio with the most potent antiproliferative activities were used in xenograft mice injected with MDA-MB-231 cell lines for in vivo tumourigenicity study as well as protein and gene expression studies. M1S9, specifically comprised of saponin and amino acid, retained the lowest antioxidant activity and highest glucosinolates content as compared to other ratios. Cell viability decreased significantly in MCF-7 breast cancer cells and PMEC after treatment with M1S9. Solid tumour from MDA-MB-231 xenograft mice was inhibited up to 64.5% at third week after treatment with high-dose M1S9. High-dose M1S9 significantly decreased the expression of calcineurin (CaN) and vascular endothelial cell growth factor (VEGF) proteins as well as the secreted frizzled-related protein 1 (SFRP1) and solute carrier family 39 member 6 (SLC39A6) genes. This study provides new scientific evidence for the chemoprevention properties of MOLSr in breast cancer model.

Subject Areas

moringa leaves and seed residue; chemoprevention; MCF-7; MDA-MB-231; gene expression profiling

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