Version 1
: Received: 2 June 2020 / Approved: 4 June 2020 / Online: 4 June 2020 (06:03:16 CEST)
How to cite:
Javalkote, V.S.; Kancharla, N.; Bhadra, B.; Shukla, M.; Soni, B.; Sapre, A.; Goodin, M.; Bandyopadhyay, A.; Dasgupta, S. CRISPR-Based Assays for Rapid Detection of SARS-CoV-2. Preprints2020, 2020060025. https://doi.org/10.20944/preprints202006.0025.v1
Javalkote, V.S.; Kancharla, N.; Bhadra, B.; Shukla, M.; Soni, B.; Sapre, A.; Goodin, M.; Bandyopadhyay, A.; Dasgupta, S. CRISPR-Based Assays for Rapid Detection of SARS-CoV-2. Preprints 2020, 2020060025. https://doi.org/10.20944/preprints202006.0025.v1
Javalkote, V.S.; Kancharla, N.; Bhadra, B.; Shukla, M.; Soni, B.; Sapre, A.; Goodin, M.; Bandyopadhyay, A.; Dasgupta, S. CRISPR-Based Assays for Rapid Detection of SARS-CoV-2. Preprints2020, 2020060025. https://doi.org/10.20944/preprints202006.0025.v1
APA Style
Javalkote, V.S., Kancharla, N., Bhadra, B., Shukla, M., Soni, B., Sapre, A., Goodin, M., Bandyopadhyay, A., & Dasgupta, S. (2020). CRISPR-Based Assays for Rapid Detection of SARS-CoV-2. Preprints. https://doi.org/10.20944/preprints202006.0025.v1
Chicago/Turabian Style
Javalkote, V.S., Anindya Bandyopadhyay and Santanu Dasgupta. 2020 "CRISPR-Based Assays for Rapid Detection of SARS-CoV-2" Preprints. https://doi.org/10.20944/preprints202006.0025.v1
Abstract
COVID-19 represents an unprecedented threat to global public health and economies. Assays are urgently needed for rapid detection and diagnosis of SARS-CoV-2-infected patients in order to inform treatment and quarantine strategies. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid-based tests are considered to be more sensitive with respect to serological tests, but the present gold standard RT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. Novel CRISPR- and other nuclease-based techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here we discuss and present compilation of state-of-the-art CRISPR based detection techniques for use in COVID diagnosis and epidemiology.
Keywords
CRISPR; Covid detection
Subject
Medicine and Pharmacology, Medicine and Pharmacology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
5 June 2020
Commenter:
Pravin Puranik
The commenter has declared there is no conflict of interests.
Comment:
I have read the nicely written article and happy to see the state-of-art information on rapid detection methods for COVID-19. This articles certainly useful to scientific as well as general readers. Reading this people will understand the difficulties and hurdles in detection of COVID-19. I wish this article reaches high number of readers and help to understand the COVID-19 issues.
Commenter: Pravin Puranik
The commenter has declared there is no conflict of interests.
Commenter:
The commenter has declared there is no conflict of interests.
Thank you so much for comment.