Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

CRISPR-Based Assays for Rapid Detection of SARS-CoV-2

Version 1 : Received: 2 June 2020 / Approved: 4 June 2020 / Online: 4 June 2020 (06:03:16 CEST)

How to cite: Javalkote, V.S.; Kancharla, N.; Bhadra, B.; Shukla, M.; Soni, B.; Sapre, A.; Goodin, M.; Bandyopadhyay, A.; Dasgupta, S. CRISPR-Based Assays for Rapid Detection of SARS-CoV-2. Preprints 2020, 2020060025 (doi: 10.20944/preprints202006.0025.v1). Javalkote, V.S.; Kancharla, N.; Bhadra, B.; Shukla, M.; Soni, B.; Sapre, A.; Goodin, M.; Bandyopadhyay, A.; Dasgupta, S. CRISPR-Based Assays for Rapid Detection of SARS-CoV-2. Preprints 2020, 2020060025 (doi: 10.20944/preprints202006.0025.v1).

Abstract

COVID-19 represents an unprecedented threat to global public health and economies. Assays are urgently needed for rapid detection and diagnosis of SARS-CoV-2-infected patients in order to inform treatment and quarantine strategies. Establishing globally accepted easy-to-access diagnostic tests is extremely important to understanding the epidemiology of the present pandemic. While nucleic acid-based tests are considered to be more sensitive with respect to serological tests, but the present gold standard RT-PCR-based assays possess limitations such as low sample throughput, requirement for sophisticated reagents and instrumentation. To overcome these shortcomings, recent efforts of incorporating LAMP-based isothermal detection, and minimizing the number of reagents required are on rise. Novel CRISPR- and other nuclease-based techniques, when merge with isothermal and allied technologies, promises to provide sensitive and rapid detection of SARS-CoV-2 nucleic acids. Here we discuss and present compilation of state-of-the-art CRISPR based detection techniques for use in COVID diagnosis and epidemiology.

Subject Areas

CRISPR; Covid detection

Comments (2)

Comment 1
Received: 5 June 2020
Commenter: Pravin Puranik
The commenter has declared there is no conflict of interests.
Comment: I have read the nicely written article and happy to see the state-of-art information on rapid detection methods for COVID-19. This articles certainly useful to scientific as well as general readers. Reading this people will understand the difficulties and hurdles in detection of COVID-19. I wish this article reaches high number of readers and help to understand the COVID-19 issues.
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Response 1 to Comment 1
Received: 7 June 2020
Commenter: Santanu Dasgupta
The commenter has declared there is no conflict of interests.
Comment: T== Heading == Thank you so much for comment.

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