Preprint Article Version 1 This version is not peer-reviewed

Inactivated Platelet Lysate Supports Proliferation and Immunomodulant Characteristics Of Mesenchymal Stromal Cells in GMP Culture Condition

Version 1 : Received: 26 May 2020 / Approved: 27 May 2020 / Online: 27 May 2020 (08:15:13 CEST)

How to cite: Mareschi, K.; Castiglia, S.; Adamini, A.; Rustichelli, D.; Marini, E.; Banche Niclot, A.G.S.; Bergallo, M.; Labanca, L.; Ferrero, I.; Fagioli, F. Inactivated Platelet Lysate Supports Proliferation and Immunomodulant Characteristics Of Mesenchymal Stromal Cells in GMP Culture Condition. Preprints 2020, 2020050443 (doi: 10.20944/preprints202005.0443.v1). Mareschi, K.; Castiglia, S.; Adamini, A.; Rustichelli, D.; Marini, E.; Banche Niclot, A.G.S.; Bergallo, M.; Labanca, L.; Ferrero, I.; Fagioli, F. Inactivated Platelet Lysate Supports Proliferation and Immunomodulant Characteristics Of Mesenchymal Stromal Cells in GMP Culture Condition. Preprints 2020, 2020050443 (doi: 10.20944/preprints202005.0443.v1).

Abstract

For their clinical use Mesenchymal Stromal Cells (MSCs), isolated from bone marrow (BM-MSCs) are considered Advanced Therapy Medicinal Products (ATMP) and need to be produced according to Good Manufacturing Practice (GMP). Human platelet lysate (HPL) represents a good GMP-compliant alternative to animal serum and after pathogen inactivation with Psoralen was more efficient and safer to produce MSCs in GMP. In this study MSCs cultivated in FBS (FBS-MSC) or inactivated HPL (iHPL-MSC), were compared for their immunomodulant properties. In particular, the effects of MSCs on: 1)proliferation of total Lymphocytes (Ly) and on naïve T Ly subsets induced to differentiate versus Th1 and Th2 Ly; 2) the immunophenotype of different T cell subsets; 3)the cytokine release to verify Th1, Th2 and Th17 polarization were analyzed by using in vitro co-culture system. We observed that iHPL-MSCs showed the same immunomodulant properties observed in the FBS-MSCs co-cultures. Although, a more efficient effect on the increase of naïve T cells and, in the Th1 cytokine release related to iHPL was observed. This study confirms that iHPL, used as medium supplement, may be considered a good alternative to FBS for a GMP-compliant MSC expansion to preserve their immunomodulant proprieties.

Subject Areas

Mesenchymal Stromal Cells; Good Manufacturing Practice; Inactivated Platelet Lysate

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