Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Inactivated Platelet Lysate Supports Proliferation and Immunomodulant Characteristics Of Mesenchymal Stromal Cells in GMP Culture Condition

Katia Mareschi
* ORCID logo ,
Sara Castiglia
,
Aloe Adamini
,
Deborah Rustichelli
,
Elena Marini
,
Alessia Giovanna Santa Banche Niclot
,
Massimiliano Bergallo
,
Luciana Labanca
,
Ivana Ferrero
,
Franca Fagioli
Version 1 : Received: 26 May 2020 / Approved: 27 May 2020 / Online: 27 May 2020 (08:15:13 CEST)

A peer-reviewed article of this Preprint also exists.

Mareschi, K.; Castiglia, S.; Adamini, A.; Rustichelli, D.; Marini, E.; Banche Niclot, A.G.S.; Bergallo, M.; Labanca, L.; Ferrero, I.; Fagioli, F. Inactivated Platelet Lysate Supports the Proliferation and Immunomodulant Characteristics of Mesenchymal Stromal Cells in GMP Culture Conditions. Biomedicines 2020, 8, 220. Mareschi, K.; Castiglia, S.; Adamini, A.; Rustichelli, D.; Marini, E.; Banche Niclot, A.G.S.; Bergallo, M.; Labanca, L.; Ferrero, I.; Fagioli, F. Inactivated Platelet Lysate Supports the Proliferation and Immunomodulant Characteristics of Mesenchymal Stromal Cells in GMP Culture Conditions. Biomedicines 2020, 8, 220.

Abstract

For their clinical use Mesenchymal Stromal Cells (MSCs), isolated from bone marrow (BM-MSCs) are considered Advanced Therapy Medicinal Products (ATMP) and need to be produced according to Good Manufacturing Practice (GMP). Human platelet lysate (HPL) represents a good GMP-compliant alternative to animal serum and after pathogen inactivation with Psoralen was more efficient and safer to produce MSCs in GMP. In this study MSCs cultivated in FBS (FBS-MSC) or inactivated HPL (iHPL-MSC), were compared for their immunomodulant properties. In particular, the effects of MSCs on: 1)proliferation of total Lymphocytes (Ly) and on naïve T Ly subsets induced to differentiate versus Th1 and Th2 Ly; 2) the immunophenotype of different T cell subsets; 3)the cytokine release to verify Th1, Th2 and Th17 polarization were analyzed by using in vitro co-culture system. We observed that iHPL-MSCs showed the same immunomodulant properties observed in the FBS-MSCs co-cultures. Although, a more efficient effect on the increase of naïve T cells and, in the Th1 cytokine release related to iHPL was observed. This study confirms that iHPL, used as medium supplement, may be considered a good alternative to FBS for a GMP-compliant MSC expansion to preserve their immunomodulant proprieties.

Keywords

Mesenchymal Stromal Cells; Good Manufacturing Practice; Inactivated Platelet Lysate

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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