Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In Vitro Evaluation of the Effects of Prebiotics GOS and FOS on Human Colonic Caco–2 Cells

Version 1 : Received: 6 April 2020 / Approved: 7 April 2020 / Online: 7 April 2020 (13:37:18 CEST)

A peer-reviewed article of this Preprint also exists.

Lafontaine, G.M.F.; Fish, N.M.; Connerton, I.F. In Vitro Evaluation of the Effects of Commercial Prebiotic GOS and FOS Products on Human Colonic Caco–2 Cells. Nutrients 2020, 12, 1281. Lafontaine, G.M.F.; Fish, N.M.; Connerton, I.F. In Vitro Evaluation of the Effects of Commercial Prebiotic GOS and FOS Products on Human Colonic Caco–2 Cells. Nutrients 2020, 12, 1281.

Journal reference: Nutrients 2020, 12, 1281
DOI: 10.3390/nu12051281

Abstract

Prebiotic oligosaccharides are widely used as human and animal feed additives for their beneficial effects on the gut microbiota. However, there are limited data to assess the direct effect of such functional foods on the transcriptome of intestinal epithelial cells. The purpose of this study is to describe the differential transcriptomes and cellular pathways of colonic cells directly exposed to galacto-oligosaccharides (GOS) and fructo-oligosaccharides (FOS). We have examined the differential gene expression of polarized Caco-2 cells treated with GOS or FOS and their respective mock-treated cells using mRNA sequencing (RNA-seq). A total of 89 significant differentially expressed genes were identified between GOS and mock-treated groups. For FOS treatment, a reduced number of 12 significant genes were observed to be differentially expressed relative to the control group. KEGG and Gene Ontology functional analysis revealed that genes up-regulated in the presence of GOS were involved in digestion and absorption processes, fatty acids and steroids metabolism, potential antimicrobial proteins, energy-dependent and -independent transmembrane trafficking of solutes and amino acids. Using our data, we have established complementary non-prebiotic modes of action for these frequently used dietary fibers.

Subject Areas

prebiotics; oligosaccharides; GOS; FOS; RNA-seq; transcriptome; differential gene expression; functional pathway analysis; Caco-2; polarized monolayers

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