A Flexible Genome-Scale SARS-CoV-2 Clone Resource

Dae-Kyum Kim; Jennifer J. Knapp; Da Kuang; Aditya Chawla; Patricia Cassonnet; Hunsang Lee; Dayag Sheykhkarimli; Payman Samavarchi-Tehrani; Hala Abdouni; Ashyad Rayhan; Oxana Pogoutse; Etienne Coyaud; Sylvie van der Werf; Caroline Demeret; Anne-Claude Gingras; Mikko Taipale; Brian Raught; Yves Jacob; Frederick P. Roth

doi:10.20944/preprints202004.0009.v4

Preprint Brief Report Version 4 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 31 March 2020 / Approved: 2 April 2020 / Online: 2 April 2020 (04:15:54 CEST)
Version 2 : Received: 3 May 2020 / Approved: 4 May 2020 / Online: 4 May 2020 (18:28:01 CEST)
Version 3 : Received: 16 June 2020 / Approved: 16 June 2020 / Online: 16 June 2020 (09:37:50 CEST)
Version 4 : Received: 19 June 2020 / Approved: 21 June 2020 / Online: 21 June 2020 (15:38:18 CEST)
Version 5 : Received: 7 July 2020 / Approved: 7 July 2020 / Online: 7 July 2020 (18:09:21 CEST)

The world is facing a major health crisis, the global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus, for which no approved antiviral agents or vaccines are currently available. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available via Addgene. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

SARS-CoV-2; coding sequence collection; gateway-compatible

Medicine and Pharmacology, Pathology and Pathobiology

Comment 1

Received: 21 June 2020
Commenter: Da Kuang
Commenter's Conflict of Interests: Author

Comment: Added missing links to Addgene and updated Table 1 to summarize the status of clones in the collection.

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A Flexible Genome-Scale SARS-CoV-2 Clone Resource

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