Version 1
: Received: 27 February 2020 / Approved: 28 February 2020 / Online: 28 February 2020 (12:03:13 CET)
How to cite:
Kim, S.; Kim, D.; Lee, B. Insufficient Sensitivity of RNA Dependent RNA Polymerase Gene of SARS-CoV-2 Viral Genome as Confirmatory Test using Korean COVID-19 Cases. Preprints2020, 2020020424. https://doi.org/10.20944/preprints202002.0424.v1
Kim, S.; Kim, D.; Lee, B. Insufficient Sensitivity of RNA Dependent RNA Polymerase Gene of SARS-CoV-2 Viral Genome as Confirmatory Test using Korean COVID-19 Cases. Preprints 2020, 2020020424. https://doi.org/10.20944/preprints202002.0424.v1
Kim, S.; Kim, D.; Lee, B. Insufficient Sensitivity of RNA Dependent RNA Polymerase Gene of SARS-CoV-2 Viral Genome as Confirmatory Test using Korean COVID-19 Cases. Preprints2020, 2020020424. https://doi.org/10.20944/preprints202002.0424.v1
APA Style
Kim, S., Kim, D., & Lee, B. (2020). Insufficient Sensitivity of RNA Dependent RNA Polymerase Gene of SARS-CoV-2 Viral Genome as Confirmatory Test using Korean COVID-19 Cases. Preprints. https://doi.org/10.20944/preprints202002.0424.v1
Chicago/Turabian Style
Kim, S., Dong-Min Kim and Baeckseung Lee. 2020 "Insufficient Sensitivity of RNA Dependent RNA Polymerase Gene of SARS-CoV-2 Viral Genome as Confirmatory Test using Korean COVID-19 Cases" Preprints. https://doi.org/10.20944/preprints202002.0424.v1
Abstract
Since mid-December of 2019, coronavirus disease 2019 (COVID-19) has been spreading from Wuhan, China. As of February 21, total 75,773 confirmed cases worldwide have spread to more than two dozen countries. Transmission of COVID-19 can occur early in the course of infection since SARS-CoV-2 viral loads in asymptomatic patients are similar to that in the symptomatic patients. Therefore, more sensitive diagnostic methods are needed to detect early phase of the infection to prevent secondary or tertiary spreads. Here, we compare the RT-PCR confirmatory test results using two different SARS-CoV-2 viral RNAs from two Korean COVID-19 confirmed cases.RT-PCR method targeting the RdRP gene, which was recommended by WHO guideline, was less sensitive than targeting N genes (as per CDC guideline). Because many countries follow the WHO guideline, our findings may contribute to the early diagnosis of COVID-19.
Keywords
SARS-CoV-2; Korean COVID-19; Wuhan Corona virus; real time PCR Ct Value; Sensitivity; False Negative
Subject
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
2 April 2020
Commenter:
Nadia Touil
The commenter has declared there is no conflict of interests.
Comment:
The article adresses a very intesting issue with the actual diagnosis of COVID-19.
How can you explain the Following profiles: Cases with negative RdRp and positive E_targeting sarbecovirus gene?
Thank you
Nadia
Commenter: sandeep
The commenter has declared there is no conflict of interests.
Commenter: Nadia Touil
The commenter has declared there is no conflict of interests.
How can you explain the Following profiles: Cases with negative RdRp and positive E_targeting sarbecovirus gene?
Thank you
Nadia
Commenter: Lee Smith
The commenter has declared there is no conflict of interests.
Interesting article but figure 1 isn't in the pdf file. Could you supply a complete version?