Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone

Version 1 : Received: 30 January 2020 / Approved: 30 January 2020 / Online: 30 January 2020 (15:06:13 CET)

A peer-reviewed article of this Preprint also exists.

Liu, K.-H.; O’Hare, D.; Thomas, J.L.; Guo, H.-Z.; Yang, C.-H.; Lee, M.-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors 2020, 10, 16. Liu, K.-H.; O’Hare, D.; Thomas, J.L.; Guo, H.-Z.; Yang, C.-H.; Lee, M.-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors 2020, 10, 16.

Journal reference: Biosensors 2020, 10, 16
DOI: 10.3390/bios10030016

Abstract

Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings, which have a great tendency to undergo covalent and hydrogen bonding interactions with similarly structured target (or template) molecules. In this work, an electrochemical method was used to optimize the synthetic self-assembly of poly(aniline-co-metanilic acid) and testosterone, forming testosterone-imprinted polymers (TIPs) on sensing electrodes. The linear sensing range for testosterone ranged from 0.1 to 100 pg/mL, and the limit of detection was as low as ~pM. Random urine samples were collected and diluted 1000 fold to measure testosterone concentration using the above TIP sensors in comparison with a commercial ARCHITECT ci 8200 system. The testosterone concentrations in the tested samples were in the range of 0.33± 0.09 to 9.13±1.33 ng/mL. The mean accuracy of the TIP-coated sensors was 90.3 ±7.0 %.

Subject Areas

testosterone; molecular imprinting; electronically conductive polymer; electrochemical sensing; urine.

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.