Liu, K.-H.; O’Hare, D.; Thomas, J.L.; Guo, H.-Z.; Yang, C.-H.; Lee, M.-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors2020, 10, 16.
Liu, K.-H.; O’Hare, D.; Thomas, J.L.; Guo, H.-Z.; Yang, C.-H.; Lee, M.-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors 2020, 10, 16.
Liu, K.-H.; O’Hare, D.; Thomas, J.L.; Guo, H.-Z.; Yang, C.-H.; Lee, M.-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors2020, 10, 16.
Liu, K.-H.; O’Hare, D.; Thomas, J.L.; Guo, H.-Z.; Yang, C.-H.; Lee, M.-H. Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors 2020, 10, 16.
Abstract
Molecularly imprinted polymers (MIPs) can often bind target molecules with high selectivity and specificity. When used as MIPs, conductive polymers may have unique binding capabilities; they often contain aromatic rings, which have a great tendency to undergo covalent and hydrogen bonding interactions with similarly structured target (or template) molecules. In this work, an electrochemical method was used to optimize the synthetic self-assembly of poly(aniline-co-metanilic acid) and testosterone, forming testosterone-imprinted polymers (TIPs) on sensing electrodes. The linear sensing range for testosterone ranged from 0.1 to 100 pg/mL, and the limit of detection was as low as ~pM. Random urine samples were collected and diluted 1000 fold to measure testosterone concentration using the above TIP sensors in comparison with a commercial ARCHITECT ci 8200 system. The testosterone concentrations in the tested samples were in the range of 0.33± 0.09 to 9.13±1.33 ng/mL. The mean accuracy of the TIP-coated sensors was 90.3 ±7.0 %.
Chemistry and Materials Science, Surfaces, Coatings and Films
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