Working Paper Article Version 1 This version is not peer-reviewed

High-frequency Near-infrared Diode Laser Irradiation Attenuates IL-1β-induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes

Version 1 : Received: 8 January 2020 / Approved: 9 January 2020 / Online: 9 January 2020 (12:22:18 CET)

How to cite: Sakata, S.; Kunimatsu, R.; Tsuka, Y.; Nakatani, A.; Hiraki, T.; Gunji, H.; Hirose, N.; Yanoshita, M.; Tanimoto, K.; Putranti, N.A.R. High-frequency Near-infrared Diode Laser Irradiation Attenuates IL-1β-induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes. Preprints 2020, 2020010086 Sakata, S.; Kunimatsu, R.; Tsuka, Y.; Nakatani, A.; Hiraki, T.; Gunji, H.; Hirose, N.; Yanoshita, M.; Tanimoto, K.; Putranti, N.A.R. High-frequency Near-infrared Diode Laser Irradiation Attenuates IL-1β-induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes. Preprints 2020, 2020010086

Abstract

High-frequency near-infrared diode laser provides high peak output, low heat accumulation, and efficient biostimulation. Although these characteristics are considered suitable for osteoarthritis (OA) treatment, the effect of high-frequency near-infrared diode laser in in vitro or in vivo OA models has not yet been reported. Therefore, we aimed to assess the biological effects of high-frequency near-infrared diode laser irradiation on IL-1β-induced chondrocyte inflammation in an in vitro OA model. Normal Human Articular Chondrocyte-Knee (NHAC-Kn) cells were stimulated with human recombinant IL-1β and irradiated with high-frequency near-infrared diode laser (910 nm, 4 or 8 J/cm2). The mRNA and protein expression of relevant inflammation- and cartilage destruction-related proteins was analyzed. IL-1β treatment significantly increased the mRNA levels of IL-1β, IL-6, TNF-α, MMP-1, MMP-3, and MMP-13. High-frequency near-infrared diode laser irradiation significantly reduced the IL-1β-induced expression of IL-1β, IL-6, TNF-α, MMP-1, and MMP-3. Similarly, high-frequency near-infrared diode laser irradiation decreased the IL-1β-induced increase in protein expression and secreted levels of MMP-1 and MMP-3. These results highlight the therapeutic potential of high-frequency near-infrared diode laser in OA.

Subject Areas

high-frequency near-infrared diode laser; osteoarthritis; inflammation; matrix metalloproteinase; human chondrocyte

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