Working PaperArticleVersion 1This version is not peer-reviewed
High-frequency Near-infrared Diode Laser Irradiation Attenuates IL-1β-induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes
Sakata, S.; Kunimatsu, R.; Tsuka, Y.; Nakatani, A.; Hiraki, T.; Gunji, H.; Hirose, N.; Yanoshita, M.; Tanimoto, K.; Putranti, N.A.R. High-frequency Near-infrared Diode Laser Irradiation Attenuates IL-1β-induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes. Preprints2020, 2020010086
APA Style
Sakata, S., Kunimatsu, R., Tsuka, Y., Nakatani, A., Hiraki, T., Gunji, H., Hirose, N., Yanoshita, M., Tanimoto, K., & Putranti, N.A.R. (2020). High-frequency Near-infrared Diode Laser Irradiation Attenuates IL-1β-induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes. Preprints. https://doi.org/
Chicago/Turabian Style
Sakata, S., Kotaro Tanimoto and Nurul Aisyah Rizky Putranti. 2020 "High-frequency Near-infrared Diode Laser Irradiation Attenuates IL-1β-induced Expression of Inflammatory Cytokines and Matrix Metalloproteinases in Human Primary Chondrocytes" Preprints. https://doi.org/
Abstract
High-frequency near-infrared diode laser provides high peak output, low heat accumulation, and efficient biostimulation. Although these characteristics are considered suitable for osteoarthritis (OA) treatment, the effect of high-frequency near-infrared diode laser in in vitro or in vivo OA models has not yet been reported. Therefore, we aimed to assess the biological effects of high-frequency near-infrared diode laser irradiation on IL-1β-induced chondrocyte inflammation in an in vitro OA model. Normal Human Articular Chondrocyte-Knee (NHAC-Kn) cells were stimulated with human recombinant IL-1β and irradiated with high-frequency near-infrared diode laser (910 nm, 4 or 8 J/cm2). The mRNA and protein expression of relevant inflammation- and cartilage destruction-related proteins was analyzed. IL-1β treatment significantly increased the mRNA levels of IL-1β, IL-6, TNF-α, MMP-1, MMP-3, and MMP-13. High-frequency near-infrared diode laser irradiation significantly reduced the IL-1β-induced expression of IL-1β, IL-6, TNF-α, MMP-1, and MMP-3. Similarly, high-frequency near-infrared diode laser irradiation decreased the IL-1β-induced increase in protein expression and secreted levels of MMP-1 and MMP-3. These results highlight the therapeutic potential of high-frequency near-infrared diode laser in OA.
Keywords
high-frequency near-infrared diode laser; osteoarthritis; inflammation; matrix metalloproteinase; human chondrocyte
Subject
Chemistry and Materials Science, Medicinal Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
