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3,4-DHPEA-EA Peracetylated (3,4-DHPEA-EA(P)) Attenuates H2O2-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway
Nardi, M.; Bardelli, S.; Ciriolo, M.R.; Colica, C.; Costanzo, P.; Procopio, A. 3,4-DHPEA-EA Peracetylated (3,4-DHPEA-EA(P)) Attenuates H2O2-Mediated Cytotoxicity in C2C12 Myocytes via Inactivation of p-JNK/p-c-Jun Signaling Pathway. Preprints2019, 2019120387. https://doi.org/10.20944/preprints201912.0387.v1
APA Style
Nardi, M., Bardelli, S., Ciriolo, M.R., Colica, C., Costanzo, P., & Procopio, A. (2019). 3,4-DHPEA-EA Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes <em>via</em> Inactivation of p-JNK/p-c-Jun Signaling Pathway. Preprints. https://doi.org/10.20944/preprints201912.0387.v1
Chicago/Turabian Style
Nardi, M., Paola Costanzo and Antonio Procopio. 2019 "3,4-DHPEA-EA Peracetylated (3,4-DHPEA-EA(P)) Attenuates H<sub>2</sub>O<sub>2</sub>-Mediated Cytotoxicity in C2C12 Myocytes <em>via</em> Inactivation of p-JNK/p-c-Jun Signaling Pathway" Preprints. https://doi.org/10.20944/preprints201912.0387.v1
Abstract
Oleuropein, glycosylated secoiridoid present in the olive leaves, is known as an important antioxidant phenolic compound. We studied the antioxidant effect of low dose of oleuropein aglycone (3,4-DHPEA-EA) and oleuropein aglycone peracetylated (3,4-DHPEA-EA(P)) in murine C2C12 myocytes treated with hydrogen peroxide (H2O2). Both compounds were used at a concentration of 10 μM and were able to inhibit cell death induced by H2O2 treatment, with 3,4-DHPEA-EA(P) being more. Under our experimental conditions H2O2 efficiently induced the phosphorylated-active form of JNK and of its downstream target c-Jun. We demonstrated, by Western blot analysis, that 3,4-DHPEA-EA(P) was efficient in inhibiting the phospho-active form of JNK. This data suggest that growth arrest and cell dead of C2C12 proceeds via the JNK/c-Jun pathway. Moreover, we demonstrated that 3,4-DHPEA-EA(P) affects myogenesis of C2C12 cells; because the MyoD mRNA levels and the differentiation process are restored after treatment with 3,4-DHPEA-EA(P). Overall, the results indicate that 3,4-DHPEA-EA(P) prevents ROS-mediated degenerative process by functioning as an efficient antioxidant.
Chemistry and Materials Science, Medicinal Chemistry
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