Preprint Article Version 3 This version is not peer-reviewed

NOS-2 Participates in the Behavioral Effects of Ethanol Withdrawal in Zebrafish

Version 1 : Received: 13 December 2019 / Approved: 16 December 2019 / Online: 16 December 2019 (11:54:10 CET)
Version 2 : Received: 31 January 2020 / Approved: 3 February 2020 / Online: 3 February 2020 (09:37:33 CET)
Version 3 : Received: 27 March 2020 / Approved: 2 April 2020 / Online: 2 April 2020 (11:01:26 CEST)

How to cite: Chaves, S.N.D.S.; Costa, B.P.D.; Gomes, G.C.V.; Lima-Maximino, M.; Rico, E.P.; Maximino, C. NOS-2 Participates in the Behavioral Effects of Ethanol Withdrawal in Zebrafish. Preprints 2019, 2019120219 (doi: 10.20944/preprints201912.0219.v3). Chaves, S.N.D.S.; Costa, B.P.D.; Gomes, G.C.V.; Lima-Maximino, M.; Rico, E.P.; Maximino, C. NOS-2 Participates in the Behavioral Effects of Ethanol Withdrawal in Zebrafish. Preprints 2019, 2019120219 (doi: 10.20944/preprints201912.0219.v3).

Abstract

Nitric oxide has been implicated in symptoms of ethanol withdrawal in animal models. Zebrafish have been used as models to study neurobehavioral effects of ethanol (EtOH) withdrawal, but the mechanisms associated with these effects are not yet clear. Adult zebrafish were treated with 1% EtOH for 20 min per day for 8 days, injected with the nitric oxide synthase 2 (NOS-2) inhibitor aminoguanidine (50 mg/kg), and allowed to experience withdrawal (WD) in their hometanks for 7 days. EtOH WD increased anxiety-like behavior in the novel tank test, an effect that was blocked by aminoguanidine. EtOH WD also increased brain levels of nitrite, an effect that was partially blocked by aminoguanidine. These results underline a novel mechanism by which NOS-2 controls anxiety-like responses to ethanol withdrawal, with implications for the mechanistic study of symptoms associated with chronic ethanol abuse.

Supplementary and Associated Material

Subject Areas

Nitric oxide; Oxidative stress; Ethanol withdrawal; Anxiety

Comments (1)

Comment 1
Received: 2 April 2020
Commenter: Caio Maximino
Commenter's Conflict of Interests: Author
Comment: Changes were made in the Methods section (including more detail on sample size calculations and improved description of tank sizes)
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