Version 1
: Received: 13 December 2019 / Approved: 16 December 2019 / Online: 16 December 2019 (11:54:10 CET)
Version 2
: Received: 31 January 2020 / Approved: 3 February 2020 / Online: 3 February 2020 (09:37:33 CET)
Version 3
: Received: 27 March 2020 / Approved: 2 April 2020 / Online: 2 April 2020 (11:01:26 CEST)
da Silva Chaves, S. N., GC, V. G., Lima-Maximino, M., & Maximino, C. (2020). NOS-2 participates in the behavioral effects of ethanol withdrawal in zebrafish. Neuroscience Letters, 728, 134952-134952.
da Silva Chaves, S. N., GC, V. G., Lima-Maximino, M., & Maximino, C. (2020). NOS-2 participates in the behavioral effects of ethanol withdrawal in zebrafish. Neuroscience Letters, 728, 134952-134952.
da Silva Chaves, S. N., GC, V. G., Lima-Maximino, M., & Maximino, C. (2020). NOS-2 participates in the behavioral effects of ethanol withdrawal in zebrafish. Neuroscience Letters, 728, 134952-134952.
da Silva Chaves, S. N., GC, V. G., Lima-Maximino, M., & Maximino, C. (2020). NOS-2 participates in the behavioral effects of ethanol withdrawal in zebrafish. Neuroscience Letters, 728, 134952-134952.
Abstract
Nitric oxide has been implicated in symptoms of ethanol withdrawal in animal models. Zebrafish have been used as models to study neurobehavioral effects of ethanol (EtOH) withdrawal, but the mechanisms associated with these effects are not yet clear. Adult zebrafish were treated with 1% EtOH for 20 min per day for 8 days, injected with the nitric oxide synthase 2 (NOS-2) inhibitor aminoguanidine (50 mg/kg), and allowed to experience withdrawal (WD) in their hometanks for 7 days. EtOH WD increased anxiety-like behavior in the novel tank test, an effect that was blocked by aminoguanidine. EtOH WD also increased brain levels of nitrite, an effect that was partially blocked by aminoguanidine. These results underline a novel mechanism by which NOS-2 controls anxiety-like responses to ethanol withdrawal, with implications for the mechanistic study of symptoms associated with chronic ethanol abuse.
Medicine and Pharmacology, Neuroscience and Neurology
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