Working Paper Review Version 1 This version is not peer-reviewed

Calmodulin and Its Binding Proteins in Dictyostelium: A Primer

Version 1 : Received: 13 December 2019 / Approved: 15 December 2019 / Online: 15 December 2019 (15:44:35 CET)

How to cite: O'Day, D.; Taylor, R.J.; Myre, M.A. Calmodulin and Its Binding Proteins in Dictyostelium: A Primer. Preprints 2019, 2019120198 O'Day, D.; Taylor, R.J.; Myre, M.A. Calmodulin and Its Binding Proteins in Dictyostelium: A Primer. Preprints 2019, 2019120198

Abstract

Dictyostelium discoideum is gaining increasing attention as a model organism for the study of calcium binding and calmodulin function in basic biological events as well as human diseases. After a short overview of calcium-binding proteins, the structure of Dictyostelium calmodulin and the conformational changes effected by calcium ion binding to its four EF hands is compared to its human counterpart, emphasizing the highly conserved nature of this central regulatory protein. The calcium-dependent and -independent motifs involved in calmodulin binding to target proteins are discussed with examples of the diversity of calmodulin binding proteins that have been studied in this amoebozoan. The methods used to identify and characterize calmodulin binding proteins is covered followed by the ways Dictyostelium is currently being used as a system to study several neurodegenerative diseases and how it could serve as a model for studying calmodulinopathies such as those associated with specific types of heart arrythmia. Because of its rapid developmental cycles, its genetic tractability, and a richly endowed stock center, Dictyostelium is in a position to become a leader in the field of calmodulin research.

Subject Areas

calmodulin; calmodulin binding proteins; calmodulin binding motifs; calcium signalling; EF hands; heart arrythmia; neurodegeneration; Dictyostelium discoideum

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