Preprint Article Version 1 This version is not peer-reviewed

Nano-Nutrition to Chicken at Different Embryonic Development Stages: Using Conjugated L-Arginine with Ag NPs to Improve Muscle Growth and Immune Related Proteins

Version 1 : Received: 13 December 2019 / Approved: 15 December 2019 / Online: 15 December 2019 (13:37:00 CET)

How to cite: Subramaniyan, S.; Da Rae, K.; Siddiqui, S.H.; Park, J.R.; Tian, W.; Park, B.; Shim, K.S. Nano-Nutrition to Chicken at Different Embryonic Development Stages: Using Conjugated L-Arginine with Ag NPs to Improve Muscle Growth and Immune Related Proteins. Preprints 2019, 2019120189 (doi: 10.20944/preprints201912.0189.v1). Subramaniyan, S.; Da Rae, K.; Siddiqui, S.H.; Park, J.R.; Tian, W.; Park, B.; Shim, K.S. Nano-Nutrition to Chicken at Different Embryonic Development Stages: Using Conjugated L-Arginine with Ag NPs to Improve Muscle Growth and Immune Related Proteins. Preprints 2019, 2019120189 (doi: 10.20944/preprints201912.0189.v1).

Abstract

The aim of the study was to analyze the in ovo injection of chemically and biologically synthesized silver nano-particles (Ag NPs) using Brassica oleracea L. var capitate f. Rubra, (BOL) conjugation with L-Arginine (L-Arg) on the immune, muscle growth, survivability and hatchability of the broiler chickens. L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) were injected into fertile eggs at 8, 14 and 18 of incubation. Survival and hatched chicks were calculated. Survivability and hatchability were unaffected by the injected dose of L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) but it significantly improved when the eggs were injected on day 14 of incubation compared with those injected on days 8 or 18. Moreover, the protein expression of muscle development markers such as myogenin and myoD were significantly up-related in 14 d incubation whereas the heat shock proteins (HSPs) such as HSP-60 and HSP-70 were significantly up-regulated in 18 d incubation. In addition, the in ovo injection on 18 d significantly increased the serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) as well the immunoglobulin (IgM) levels were increased in 14-day incubation period in serum at the same concentration. Collectively, these results indicate that injecting L-Arg (100 μg) with 1000 µg of BOL-Ag NPs or L-Arg (100 μg) with 100 µg of C-Ag NPs to eggs at day 14 after incubation could improve their hatchability, survival rate, and muscle growth marker.

Subject Areas

nanoparticles; silver; L-arginine; conjugation; muscle growth

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